HPLC法测定复方呋喃西林滴鼻液中盐酸麻黄碱的含量  

Determination of ephedrine hydrochloride in compound furacilin nasal drops by HPLC

作　　者：王逢春[1] 王敏[2] 张瑞麟[3] 

机构地区：[1]北京军区总医院医务处,北京100700 [2]北京军区总医院263临床部医务处,北京101149 [3]空军总医院药学部,北京100142

出　　处：《实用药物与临床》2014年第1期71-73,共3页Practical Pharmacy and Clinical Remedies

摘　　要：目的 建立高效液相色谱法测定复方呋喃西林滴鼻液中盐酸麻黄碱的含量.方法 色谱柱：Agilent Zorbax SB-C18（4.6 mm × 250 mm,5 μm）;流动相：甲醇-0.05%磷酸（35：65）（每100 mL加十二烷基磺酸钠0.1 g）;流速：1.0 mL/min;检测波长：210 nm;柱温：30 ℃.结果 盐酸麻黄碱的线性范围为10.00~200.0 μg/mL.平均回收率为99.99%,RSD为0.54%（n=6）.结论 本方法 快速、简便,可提高含量测定结果 的准确性.Objective To establish an HPLC method for the determination of ephedrine hydrochloride in com- pound furacilin nasal drops. Methods Agilent Zorbax SB-C18 （4. 6 mm×250 mm, 5 txm） chromatographic column was used and the column temperature was 30~C ;the mobile phase consisted of methanol-0. 05 % phosphoric acid （35： 65 ） （0. 1 g dodecyl sodium was added into 100 mL solution） , the flow rate was 1.0 mL/min;the detective wavelength was 210 nm. Results The liner ranges of ephedrine hydrochloride was at 10. 00 - 200. 0 ixg/mL,the average recovery was 99. 99% ,RSD was 0. 54% （n =6）. Conclusion The method is rapid and simple,it can improve the accuracy of determination results of compound furacilin nasal drops.

关 键 词：复方呋喃西林滴鼻液 盐酸麻黄碱 含量测定 

分 类 号：R927[医药卫生—药学]