多指标正交试验优选体外模拟草酸钙结石模型最佳条件  

Optimization Study on Influential Factors for Simulation Calcium Oxalate Calculus Models in Vitro by Orthogonal Design with Multi-Targets

作　　者：曾春晖[1] 杨柯[1] 李先梅[1] 谭娥玉[1] 蔡妮娜[1] 徐明光[1] 

机构地区：[1]广西中医药大学药学院,南宁530001

出　　处：《世界科学技术-中医药现代化》2013年第9期1937-1940,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广西科学技术厅自然科学基金委面上项目(2011GXNSFA018204):五眼果抗上尿路结石的活性部位及其作用机制研究;负责人:杨柯;广西科学技术厅创新研究团队项目(2011GXNSFF018006):中药新药基础研究;负责人:朱华;广西卫生厅中医药科技专项课题(GZKZ10-050):五眼果对草酸钙尿石症作用的活性部位及其作用机制研究;负责人:杨柯

摘　　要：目的:选取晶体生长速率和(COM和聚集体量)/总晶体量比例为评价指标,优化体外模拟草酸钙结石模型条件。方法:采用琼脂凝胶双向扩散法,选取琼脂凝胶浓度、培养温度、培养时间、氯化钙和草酸钠配比4个因素进行正交试验,通过光学显微镜定时观察对草酸钙晶体晶型的生成,优选出最佳体外草酸钙结晶形成的条件。结果:随着培养时间的延长,不同浓度琼脂凝胶均呈现一定比例的一水草酸钙(COM)、二水草酸钙(COD)、三水草酸钙(COT)及聚集体,其中以1%琼脂浓度下的聚集速率最快且易形成COM;培养温度在37℃时最利于COM的成核、生长以及聚集;0.1/0.2 mol·L-1氯化钙/草酸钠,在37℃下培养3天,生成的COM及聚集体比例最大,达总晶体量90%以上。结论:pH7.4、1%琼脂及0.1/0.2 mol·L-1氯化钙/草酸钠的琼脂凝胶研究系统在37℃下培养3天,为复制以COM为主的体外草酸钙结石模型最佳条件。This study was aimed to optimize the influential factors on simulation calcium oxalate calculus model in vitro with the targets of the CaOxa crystal growth rate and the ratio of CaOxa crystals. The model of calcium oxalate stone agar gel system was established in order to evaluate four influential factors which include the concentration of agar gel, incubation temperature, incubation time, calcium chloride / sodium oxalate for the orthogonal test. The for- mation of calcium oxalate stone was observed under the microscope. Then, the formation conditions of calcium ox- alate stone in vitro were optimized. The results showed that as the extension of incubation time, different concentra- tions of agar gel were rendered in a certain percentage of COM, COD, COT as well as aggregates. Among them, the gathered rate was the quickest and easy to form COM under 1% agar concentration. When the cultivation con- dition was 37℃, it was the most suitable condition for COM nucleation, growth, and gather. With concentration of calcium chloride / sodium oxalate as 0.1/0.2 mol/L, incubating in 37℃ for 3 days, COM and aggregate accounted for more 90% of all crystals. It was concluded that the optimal conditions for the model of calcium oxalate stone formation in vitro were incubated in 37℃ for 3 days with 1% agar gel, calcium chloride / sodium oxalate as 0.1/ 0.2 mol/L at pH 7.4.

关 键 词：草酸钙结石 体外模型 影响因素 最佳条件 

分 类 号：R691.4[医药卫生—泌尿科学]