缺血预处理对心肌缺血再灌注诱发凋亡的保护机制  

The protective mechanism of ischemic preconditioning on apoptosis induced by myocardial ischemia reperfusion

作　　者：李上海[1] 梁伟钧[1] 孙炎华[1] 吴铿[1] 

机构地区：[1]广东医学院附属医院心内科,湛江524000

出　　处：《国际心血管病杂志》2014年第1期48-50,共3页International Journal of Cardiovascular Disease

基　　金：广东省科技计划项目(2009B030801337)

摘　　要：目的:通过观察心肌细胞凋亡指数与心肌中Fas、c-fos表达阳性率的变化,探讨缺血预处理对心肌缺血再灌注诱发细胞凋亡的保护作用及与心肌中Fas、c-fos基因表达的关系。方法:将入选大鼠随机分为假手术对照组(A组)、缺血组(B组)、缺血再灌注组(C组)、缺血预处理组(D组),每组15只,测定各组大鼠心肌中细胞凋亡和Fas、cfos的表达,并分析三者之间的相关性。结果:与A组比较,B、C及D组大鼠的心肌凋亡指数、心肌中Fas与c-fos的阳性率均升高(P<0.01);与C组比较,B组及D组大鼠的心肌凋亡指数、心肌中Fas与c-fos的阳性率均降低(P<0.01)。Pearson相关分析显示心肌凋亡指数、Fas、c-fos三者之间存在显著正相关。结论:缺血预处理可能通过下调凋亡相关基因Fas、c-fos的表达,减少细胞凋亡而发挥心肌保护作用。Objective..To detect apoptotic index （AI）,Fas and c-fos expressions after ischemic preconditioning,myocardial ischemia and reperfusion injury,and to investigate their relationship.Methods：A total of 60 SD rats were randomly divided into 4 groups as follows：sham-operation group （A）,ischemia group （B）,ischemia reperfusion group （C） and ischemia precondition group （D）.After indicated treatment,AI of myocardium was determined by TUNEL,and the expressions of Fas and c-fos were detected by immunohistochemistry.The correlation between AI,Fas and c-fos was analyzed by using Pearson correlation.Results：Compared with group A,the expressions of Fas and c-fos and AI were all increased significantly in groups B,C and D （P＜ 0.01）.And they were all decreased significantly in groups B and D compared with group C （P＜0.01）.There was a positive correlation between AI,Fas and c-fos （P ＜ 0.01）.Conclusion：Ischemic preconditioning protects myocardium against apoptosis induced by myocardial ischemia reperfusion,which may be related to the down regulation of Fas and c-fos in the myocardium.

关 键 词：缺血预处理 心肌细胞凋亡 FAS基因 C-FOS基因 

分 类 号：R541.4[医药卫生—心血管疾病]