心脑舒通及其单体成分对内毒素激活的小胶质细胞的影响  

Effect of Xinnao Shutong and its monomer on Lipopolysaccharides activated microglia cells

作　　者：卢国彦[1] 杨红云[1] 王少峡[1] 胡利民[1] 

机构地区：[1]天津中医药大学重点实验室,现代中药发现国家重点实验室,天津市中药药理学重点实验室,教育部方剂学重点实验室,天津300193

出　　处：《天津中医药大学学报》2014年第1期19-21,共3页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：重大新药创制(2011ZX09201-201)

摘　　要：[目的]研究心脑舒通及其单体成分对小胶质细胞(BV-2)的影响及其作用机制。[方法]实验分为对照组、脂多糖刺激组、加药组,检测心脑舒通(不同稀释倍数的心脑舒通及其单体成分)对小胶质细胞活力及脂多糖(LPS)诱导小胶质细胞一氧化氮(NO)产生的影响。[结果]心脑舒通对LPS诱导的小胶质细胞NO的产生无明显抑制作用,在心脑舒通13种单体成分中,伪原薯蓣皂苷、支脱皂苷元、薯蓣皂苷元可以在不影响细胞活力的情况下抑制NO产生。[结论]心脑舒通部分单体成分抑制激活小胶质细胞NO的产生可能是心脑舒通发挥神经保护作用机制之一。[Objective] To study the effects of Xinnao Shutong ana its monomer composmon on mlcroglta （BV-2） and its mechanism. [Methods] The experiment was divided into control group, lipopolysaccharide（LPS） group, dosing group. The effect of Xinnao Shutong （different dilution multiples of Xinnao Shutong and its monomer composition） on cellular activity and the production of nitric oxide （NO） in microglia cell induced by lipopolysaccharide （LPS）. [Results] Xinnao Shutong could not significantly inhibit the microglia in releasing NO induced by LPS. Among the 13 kinds of monomer composition of Xinnao Shutong, Pseudoprotodioscin, Gitogenin, Diosgenin could inhibit NO secretion without affecting the cell vitality. [Conclusion] Some monomer composition of Xinnao Shutong can inhibit the releasing of NO in activated microglia. It may be one of the mechanisms of neural protection.

关 键 词：心脑舒通 一氧化氮 炎性介质 小胶质细胞 

分 类 号：R285.5[医药卫生—中药学]