高山红景天立枯病菌菌丝融合群鉴定及药剂敏感性  

Anastomosis Group Identification and Susceptibility to Fungicides on Rhodiola sachalinensis Damping off in Jilin Province

作　　者：白庆荣[1] 谢昀烨[1] 姜旭宇 孙慧颖[1] 高洁[1] 

机构地区：[1]吉林农业大学,长春130118

出　　处：《东北林业大学学报》2014年第1期107-111,共5页Journal of Northeast Forestry University

基　　金：吉林省自然科学基金(20101565)

摘　　要：采用组织分离法从吉林省临江市、安图县罹病高山红景天植株分别获得4、2个致病菌株。病菌的形态学特征、培养性状、细胞核染色、菌丝融合及rDNA-ITS区序列测定结果表明：6个菌株均为茄立枯丝核菌（ Rhi-zoctonia solani K＆#252;hn），属多核丝核菌；临江分离物J1～J4为AG-4-HGⅡ菌丝融合群，安图分离物J5～J6为AG-1-IB菌丝融合群。19种杀菌剂对2个菌丝融合群的敏感性测定结果表明：2个菌丝融合群对蜡质芽孢杆菌、枯草芽孢杆菌、井冈·蜡芽菌的敏感性最好（EC50＜0．005 mg/L）；对多粘类芽孢杆菌和枯草芽孢杆菌（强尔）的敏感性次之（EC50＜1．0 mg/L）；对木霉菌和寡雄腐霉的敏感性略低（1．0 mg/L＜EC50＜5．0 mg/L）。2个菌丝融合群对化学杀菌剂肟菌·戊唑醇、氟硅唑、腐霉利、多菌灵敏感性较高（EC50＜1．0 mg/L）；对异菌脲、菌核净、氟菌唑、百菌清和咪鲜胺的敏感性略低（1．0 mg/L＜EC50＜10．0 mg/L）；AG-1-IB菌丝融合群对咯菌腈、噁霉灵、嘧霉胺的敏感性强于AG-4-HGⅡ。Six isolates with pathogenicity were obtained from the stems of diseased plants with tissue isolation method （ four iso-lates from Linjiang City and two isolates from Antu County）.The experiment was conducted to investigate their characteris-tics of morphology and cultivation.Rhizoctonia solani K＆#252;hn is the pathogen of the disease.Anastomosis groups are deter-mined to be AG-1-IB subgroup in Antu and AG-4-HGⅡsubgroup in Linjiang by pairing isolates with tester trains and se-quence analysis of rDNA-ITS region.By fungicides susceptibility test, two anastomosis groups are most sensitive to Bacillus cereus, B.subtilis （1011/g）, Validamycin· B.cereus （EC50〈0.005 mg/L） followed by Paenibacillus polymyxa, B.sub-tilis（108/g）（EC50〈1.0 mg/L）, Trichoderma and Pythium oligandrum （1.0 mg/L〈EC50〈5.0 mg/L）.Two anastomosis groups are most sensitive to trifloxystrobin· tebuconazole, flusilazole, procymidone and carbendazim （EC50〈1.0 mg/L） followed by iprodione, dimethachlon, triflumizole, chlorothalonil and prochloraz （1.0 mg/L〈EC50〈10.0 mg/L）.AG-1-IB is more sensitive to celest, hymexazol and hymexazol than AG-4-HGⅡ.

关 键 词：景天立枯病 病原鉴定 菌丝融合群 药剂敏感性 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]