慢性乙型肝炎湿热中阻证的血浆代谢组学  

Metabonomic analysis of plasma samples from chronic hepatitis B patients with dampheat stasis in the middle-jiao syndrome

作　　者：刘友平[1] 李洪[1] 张光海[2] 米绍平[2] 李波[2] 魏嵋[2] 

机构地区：[1]泸州医学院生物化学教研室,四川省泸州市646000 [2]泸州医学院附属中医医院肝胆内科,四川省泸州市646000

出　　处：《世界华人消化杂志》2014年第1期107-113,共7页World Chinese Journal of Digestology

基　　金：四川省教育厅科技基金资助项目;Nos.2009ZA048;13ZA0239~~

摘　　要：目的:从代谢组学的角度探讨慢性乙型肝炎湿热中阻证的分子机制.方法:采用超高性能液相色谱与质谱(UPLC-Q-TOP/MS)联用技术并结合正交偏最小二乘方-判别分析(OPLS-DA)进行对比分析慢性乙型肝炎湿热中阻证与正常对照组血浆代谢物水平.结果:慢性乙型肝炎湿热中阻证组与正常对照组有明显的代谢图谱分离;慢性乙型肝炎湿热中阻证组比较正常对照组有14种代谢产物主成分差异,其中牛磺鹅脱氧胆酸、胆烷酸、甘氨鹅脱氧胆酸-3-葡萄糖醛酸、胆酸葡萄糖醛酸、甘氨鹅脱氧胆酸-3-葡萄糖醛酸等主要与胆汁酸代谢密切相关;溶血磷脂酰胆碱(C18:3)、溶血磷脂酰胆碱(C18:2)、溶血磷脂酰乙醇胺(C22:1)、二羟基雄甾烯酮硫酸、磷脂酰胆碱、磷脂酰乙醇胺(C24:1)、13'-羟基-α-生育酚等主要与脂类代谢密切相关.结论:慢性乙型肝炎湿热中阻证患者与健康者存在代谢产物主成分差异,这些代谢产物主成分差异可能是慢性乙型肝炎湿热中阻证的潜在分子标志物.AIM： To investigate the molecular mechanisms of damp-heat stasis in the middle-jiao syndrome in patients with chronic hepatitis B. METHODS： Plasma samples were collected prospectively from normal controls （n = 20） and patients with chronic hepatitis B and damp-heat stasis in the middle-jiao syndrome （DHSS） （TM1 group： n = 20） and analyzed using ultraperformance liquid chromatography （LC）/time-of-flight mass spectrometry （MS） coupled with orthogonal partial least squares-discriminant analysis （OPLS-DA）. RESULTS： Significant metabonomic differences were observed between the two groups. Plasma levels of 14 metabolites in the TM1 group were obviously altered compared with those in the control group （P 〈 0.05）, among which taurochenodesoxycholic acid, 7b-hydroxy-3-oxo-5b-cholanoic acid, glycochenodeoxycholic acid 3-glucuronide, glycochenodeoxycholate-3-sulfate and cholic acid glucuronide are related to the metabolism of bile acids, lysophosphatidylcholine （lysoPC）, lysophosphatidylethanolamine （LysoPE） （C22：1）, 3b,16a-dihydroxyandrostenone sulfate, phosphatidylcholine （PC） （C22：6）, phosphatidylethanolamine （PE） （C24：1）, lysophosphatidylcholine （lysoPC） （C18：2） and 13＇-hydroxy-alpha-tocopherol are related to the metabolism of lipids. CONCLUSION： There are significant metabonomic differences between normal controls and patients with chronic hepatitis B and DHSS, and these metabolites may be used as potential biological markers for DHSS in patients with chronic hepatitis B.

关 键 词：慢性乙型肝炎 湿热中阻证 血浆 代谢组学 

分 类 号：R259[医药卫生—中西医结合]