透骨草提取物对人卵巢SKOV-3细胞Caspases蛋白表达的影响  

Influences of Phryma Leptostachya L. var. Asiatica Hara Extracts on Expressions of Caspase Proteins of SKOV-3 Cells

作　　者：田晶 孙峥[2] 曾常茜[3] 

机构地区：[1]图们市石岘镇中心卫生院,吉林图们133101 [2]大连医科大学,辽宁大连116044 [3]大连大学医学院,辽宁省生物有机重点实验室,辽宁大连116622

出　　处：《中医药信息》2014年第1期17-19,共3页Information on Traditional Chinese Medicine

基　　金：大连市科学技术基金计划项目(No.2010E13SF144)

摘　　要：目的:研究透骨草提取物对人卵巢SKOV-3细胞凋亡的影响及其机制。方法:吖啶橙染色法观察透骨草提取物对SKOV-3细胞形态的影响;免疫组化法检测透骨草提取物对SKOV-3细胞Caspases蛋白表达的影响。结果:37.8μg/ml透骨草提取物作用于SKOV-3细胞24h后,SKOV-3细胞形态不规则,细胞核固缩、呈月牙形紧贴在核膜周围。SKOV-3细胞Caspase-9、Caspase-3、Caspase-7蛋白表达明显低于与对照组(P<0.05),表明透骨草提取物可下调SKOV-3细胞Caspase-9、Caspase-3、Caspase-7蛋白的表达。结论:透骨草提取物可以通过Caspases依赖途径诱导SKOV-3细胞凋亡。Objective： To investigate the influences of Phryma Leptostachya L. var. asiatica Hara extracts on expressions of Caspase proteins of SKOV -3 cells and related molecular mechanism. Methods： The morph of SKOV - 3 cells was observed with acridine orange staining method. The expressions of Caspase - 9, Caspase - 3 and Caspase -7 proteins of SKOV -3 cells were detected with immunohistochemistry technique. Results： The morph of SKOV - 3 cells was unregular, karyopyenosis aggregated beside edge of SKOV - 3 cell mem- brane with crescent after SKOV -3 cells were treated with 37.81μg/ml Phryma Leptostachya L. var. asiatica Hara extracts. The expressions of Caspase - 9, Caspase - 3 and Caspase - 7 proteins of SKOV - 3 ceils treated with 37.8 p,g/ml Phryma Leptostachya L. var. asiatiea Hara extracts decreased than those in the control group （ P 〈 0. 05 ）, indicating Phryma Leptostachya L. var. asiatiea Hara extracts could downregulate expressions of Caspase -9, Caspase -3 and Caspase -7 proteins of SKOV -3 cells. Conclusion： Phryma Leptostachya L. var. asiatiea Hara extracts could induce the apoptosis of SKOV -3 cells by Caspase proteins pathway.

关 键 词：透骨草提取物 SKOV-3细胞 凋亡 CASPASE-9 CASPASE-3 CASPASE-7 

分 类 号：R285.5[医药卫生—中药学]