头针透穴法对脑出血大鼠脑组织中β-catenin表达影响的实验研究  

Effect of Scalp Penetration Acupuncture on β-catenin Expression of Brain Tissues of Rats with Cerebral Hemorrhage

作　　者：邹伟[1] 于婷婷[1] 王冬[1] 孙晓伟[1] 王珑[1] 

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《中医药信息》2014年第1期76-79,共4页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.30772840);哈尔滨市科技创新基金项目(No.3006RFXXS047);黑龙江省教育厅科学技术研究项目(No.12521497)

摘　　要：目的:通过观察针刺"百会"透"曲鬓"穴头针疗法对急性脑出血大鼠脑组织形态学及脑组织中β-catenin表达的影响,研究针刺对脑出血后NSCs增殖分化的影响及其与Wnt信号通路关键因子β-catenin的关系,阐明针刺促进脑出血后神经重塑的作用机制。方法:选用雄性Wistar大鼠按随机数字表法随机分为空白组、模型组、针刺组、通路激动剂(SB216763)组;每组再随机分为1天、3天、7天、14天4个亚组。模型组采用立体定向手术自体血注入法制备脑出血模型;针刺组大鼠针刺患侧"百会"透"曲鬓"穴。在光镜下观察各组大鼠脑组织形态学改变;运用蛋白免疫印迹方法检测和免疫组化方法检测各组大鼠脑组织β-catenin的阳性细胞表达以及针刺治疗对它们的影响。结果:针刺及激动剂组可明显改善大鼠神经功能缺损评分,与模型组比较,有显著性差异(P<0.05)。模型组、针刺组及SB216763组大鼠灶周组织β-catenin阳性细胞数表达均随时间点变化逐渐增加,3天时达高峰值,7天逐渐减少,14天时仍有表达;针刺组、SB216763组分别与模型组比较,差异均有统计学意义(P<0.05);针刺组与SB216763组中β-catenin表达比较无明显差异(P>0.05)。结论:针刺及通路激动剂均可明显改善脑出血大鼠神经损伤功能评分。"百会"透"曲鬓"针刺法可能具有与通路激活剂SB216763有相类似的作用,可能通过上调β-catenin表达,激活Wnt信号通路,参与调控脑出血大鼠脑组织中神经干细胞的增殖与分化。Objective：To observe the effect of acupuncture “Baihui” through “Qubin” head acupuncture effect on expression of β- catenin morphology of rats＇ brain tissue and brain tissue in the acute cerebral hemor- rhage, effect of acupuncture on the proliferation and differentiation of NSCs after intracerebral hemorrhage and its relationship with Wnt signal pathway key factor beta -catenin, elucidating acupuncture action mechanism to promote neural plasticity after cerebral hemorrhage. Methods： The rats were randomly divided into blank group, model group, acupuncture group, channel agonist male Wistar rats （SB216763） group; each group was randomly divided into 1 days, 3 days, 7 days, 14 days and four subgroups. The model group was by ster- eotactic operation of autologous blood preparation of cerebral hemorrhage model rats of acupuncture group acu- puncture injection ; side “Baihui” through “Qubin” point. Morphological observation of brain tissue of rats inthe changes under light microscope; using Western blotting and immunohistochemistry method to detect the effect of positive cell expression in brain tissue in rats of β - catenin and acupuncture treatment on them. Re- suits： Acupuncture and agonist group could significantly improve the neurological deficit scores of rats, com- pared with model group, there were significant differences （P 〈 0.05 ）. Immunohistochemistry showed ： model group, acupuncture group and SB216763 group β- catenin positive cells in peripheral tissues of rats with focal expression of time change gradually increased, reached the peak value at 3d, 7d decreased gradually, there when 14d expression; acupuncture group, SB216763 group and model group were compared, there was a sta- tistically significant difference （ P 〈 0.05 ） ; acupuncture group and SB216763 group 13 - catenin expression showed no significant difference （P 〉 O. 05 ）. Conclusion： Acupuncture and channel agonists could signifi- cantly improve the score function of nerve injury in rats wi

关 键 词：头针透刺法 脑出血 Β-CATENIN 

分 类 号：R245[医药卫生—针灸推拿学]