瑞舒伐他汀抑制小鼠哮喘模型慢性气道炎症反应的实验研究  

Rosuvastatin attenuates airway inflammation of chronic asthma in mice

作　　者：张维[1] 朱涛[2] 王导新[3] 邓洁 李万成[1] 

机构地区：[1]成都医学院第一附属医院,四川成都610000 [2]四川大学华西医院呼吸内科,四川成都610041 [3]重庆医科大学附属第二医院呼吸内科,重庆400010 [4]不详

出　　处：《西部医学》2014年第2期150-153,共4页Medical Journal of West China

基　　金：国家自然科学基金(30971303)

摘　　要：目的评价瑞舒伐他汀对小鼠哮喘模型气道炎症反应的影响。方法 30只雄性BALB/C小鼠随机平均分为3组,即空白组(Control组)、卵清蛋白+瑞舒伐他汀组(OVA+RUS组)和卵清蛋白组(OVA组)。在干预81天后处死小鼠获取支气管灌洗液(BALF)并使用ELISA测定IL-4、IL-5、IL-13和TNF-α含量,同时测定BALF中细胞总数、嗜酸性粒细胞、中性粒细胞、淋巴细胞和巨噬细胞数;并获得肺组织标本,进行HE染色和PAS染色及粘蛋白糖原表达测量。结果 HE染色发现,OVA致敏和激发81天后BALF中IL-4、IL-5、IL-13和TNF-α以及细胞总数、嗜酸性粒细胞、中性粒细胞、淋巴细胞和巨噬细胞数均明显增加(均P<0.05);同时小鼠肺组织出现明显的病理改变,且OVA组较OVA+RUS组更加明显(均P<0.05)。PAS染色和粘蛋白糖原表达分析发现,OVA干预后小鼠粘蛋白糖原表达明显增加,且OVA组较OVA+RUS组增加更显著(均P<0.05)。结论瑞舒伐他汀可以减轻OVA诱导的慢性哮喘导致的气道炎症反应和气道粘液高分泌,为进一步临床应用奠定了基础。Objective To investigate the treatment value of rosuvastatin （RUS） in airway inflammation in a murine model of chronic asthma. Methods Thirty male BALB/C mice were randomly divided into control group, OVA＋RUS group and OVA group （n=10）. IL-4, IL-5, IL-13 and TNF-a in BALF were measured by ELISA. Total cells, lympho- cytes, macrophages, neutrophils, and eosinophils were measured by Diff-Quik staining. HE staining was used to observe the pathological changes. PAS staining was used to detect and measure mucus secretion. Results After 81 days of OVA sensitization and challenge, IL-4, IL-5, IL-13, TNF-a, total cells, lymphocytes, macrophages, neutrophils, and eosino- phils in BALF were all significantly increased, and the obvious pathological changes were observed in mice with OVA stimulation. However, OVA group was more severe than OVA-t-RUS group. Mucus secretion was significantly in- creased after OVA stimulation as well. However, mucus secretion in OVA-t-RUS group was remarkably lower than OVA group. Conclusion Rosuvastatin could inhibit airway inflammation and mucus secretion in a murine model of chro- nic asthma.

关 键 词：瑞舒伐他汀 支气管哮喘 气道炎症反应 气道粘液高分泌 

分 类 号：R965[医药卫生—药理学] R364.5[医药卫生—药学]