替米沙坦通过上调PPAR-γ促进结肠癌SW480细胞TIMP-1表达的实验研究  

Telmisartan enhances the expression of TIMP-1 of SW480 Cells by up-regulation of PPAR-γ

作　　者：尹鹏[1] 胡君[1] 储著凌[1] 霍中华[1] 侯乐伟[1] 吕盛[1] 栾荣刚[1] 胡国强[1] 

机构地区：[1]解放军第四五四医院普外烧伤整形外科,江苏南京210002

出　　处：《西部医学》2014年第2期160-162,共3页Medical Journal of West China

摘　　要：目的探讨替米沙坦能否通过上调PPAR-γ促进结肠癌SW480细胞TIMP-1表达。方法使用替米沙坦对SW480细胞进行干预。在不同时间点(0、24和72h)使用MTT法对SW480细胞活性进行测定;使用PT-PCR对在不同时间点(0、24和72h)细胞表达的TIMP-1和PPAR-γmRNA进行测定;使用western blotting对在不同时间点(0、24和72h)细胞表达的TIMP-1和PPAR-γ蛋白进行测定。结果替米沙坦干预后SW480细胞活性逐渐降低,明显低于对照组(均P<0.05),且各时间点间的细胞活性存在显著统计学差异(均P<0.05);不同时间点(0、24和72h)TIMP-1、PPAR-γmRNA和蛋白的表达呈时间依赖性递增(均P<0.05)。结论本实验表明,替米沙坦可能是通过上调PPAR-γ的表达促进TIMP-1的合成和分泌,导致SW480细胞生长和侵袭能力的降低。Objective To investigate whether telmisartan would promote TIMP-1 via up-regulation of PPAR-γ in SW480 cells. Methods MTT assay was used to measure the cell viability. RT-PCR was included to analyze TIMP-1 and PPAR-γ mRNA. The protein expression of TIMP-1 and PPAR-γ was measured by western blotting. Results After telm- isartan intervention, the cell viabilities of SW480 were time dependently decreased. Then, the mRNA and protein expres- sion of TIMP-1 and PPAR-γ were time dependently up-regulated by telmisartan. Conclusion Telmisartan could promote TIMP-1 probably through up-regulation of PPAR-γ in SW480 cells.

关 键 词：替米沙坦 TIMP-1 PPAR-Γ SW480细胞 

分 类 号：R965.1[医药卫生—药理学]