基于分子可操作分类单元的北京地区杨树内生葡萄座腔菌属真菌多样性  

Diversity of Botryosphaeria spp.,as Endophytes in Poplars in Beijing,Based on Molecular Operational Taxonomic Units

作　　者：李如华[1] 严东辉[1] 冯小慧[1] 孙晓明[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室,北京100091

出　　处：《林业科学》2014年第1期109-115,共7页Scientia Silvae Sinicae

基　　金："十二五"国家高新技术研究和发展计划项目(863)(2012AA101501);引进国际先进林业科学技术计划项目(2013-4-10)

摘　　要：树木内生真菌的多样性是树木健康和树木与环境互作的基础,基于分子可操作分类单元的DNA条形码在揭示微生物多样性上起着重要作用。依据DNA条形码标准和技术,经由来自公共核酸数据库(NCBI)中葡萄座腔菌属及其相关属的ITS序列有效化以及对杨树皮层组织分离培养真菌ITS序列扩增和亲缘关系分析发现,该属真菌是常见的杨树内生真菌,在北京地区的杨树上存在葡萄座腔菌、红葡萄座腔菌和小葡萄座腔菌3个种群,葡萄座腔菌为优势种群,每个种群内也存在遗传分化。Diversity of endophytic fungi in trees is a key to tree health and interaction between trees and environment. Operational taxonomic units based on molecular DNA barconding an important role in disclosing microbiological diversity. Based on criteria and techniques of DNA barcoding approaches, a validation database on ITS sequences of Botryosphaeria genus and its related fungi downloaded from public nucleotide databases in NCBI,was constructed for determining the strains from endophytes in poplars in Beijing area, along with their ITS amplification and phylogenetic relationship analysis. The results showed that there were three populations of Botryosphaeria species parasitic in poplars as endophytes, B. dothidea, B. rhodina and B. parva. Strains of each population had genetica diversity. The approaches and results, presented in this paper,would contribute to a comprehensive understanding endophyte diversity in poplars and promoting researches on relationships between poplar hosts and Botryosphaeria pathogen as well as poplar canker fungal pathagenesis and control.

关 键 词：内生真菌 BOTRYOSPHAERIA DNA条形码 分类 多样性 

分 类 号：S718.81[农业科学—林学]