甘露聚糖酶多克隆抗体的制备及其ELISA双抗夹心法的建立  

Preparation of Polyclonal Antibodies of Mannanase and the Establishment of ELISA Double-antibody Sandwich Method

作　　者：范文斌[1] 王和平[2] 刘永斌[3] 

机构地区：[1]呼和浩特职业学院,内蒙古呼和浩特010051 [2]内蒙古农业大学,内蒙古呼和浩特010018 [3]内蒙古农牧业科学院,内蒙古呼和浩特010031

出　　处：《畜牧与饲料科学》2014年第1期12-14,共3页Animal Husbandry and Feed Science

摘　　要：该研究旨在建立一种检测转β-甘露聚糖酶基因大肠杆菌表达产物的有效方法。由于表达量有限及产物结构的变化,常规的检测方法不能准确地判断表达产物的性质,因此,该研究运用自制的抗原和抗血清建立酶联免疫法来进行检测。检测结果表明,转β-甘露聚糖酶基因大肠杆菌成功地表达了β-甘露聚糖酶。The purpose of this study was to establish an effective method for detecting the expressed product by Escherichia coli transformed byβ-mannanase. Due to little expression amount and the changes of product structure, the conventional detection method couldn′t accurately judge the properties of expression product. So the selfmade antigen and anti-serum were used to establish ELISA. The detection results showed that E. coli transformed byβ-mannanase could successfully expressβ-mannanase.

关 键 词：抗血清 Β-甘露聚糖酶 酶联免疫法 

分 类 号：S852.43[农业科学—基础兽医学]