DNA固定纳米金修饰的玻碳电极对腺嘌呤的测定及应用研究  

Determination of adenine by glassy carbon electrode modified with DNA-anchored gold nanoparticles

作　　者：牛凌梅[1] 张玉娜[2] 连靠奇[1] 石红梅[1] 康维钧[1] 

机构地区：[1]河北医科大学公共卫生学院,河北石家庄050017 [2]河北医科大学第四医院内分泌科,河北石家庄050011

出　　处：《中国卫生检验杂志》2014年第1期30-31,34,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金(81172722;81273128);河北省卫生厅课题项目(20130460)

摘　　要：目的建立DNA固定的双层纳米金修饰玻碳电极测定腺嘌呤的新方法。方法利用差分脉冲法（DPV）研究了腺嘌呤的电化学响应。结果发现双层纳米金复合膜对于腺嘌呤的氧化能够起到明显的电催化作用。在优化条件下，腺嘌呤的氧化峰电流与其浓度在3．0×10-8-1．0×10-7，2．0×10-7～7．0×10-7mol／L范围内呈良好的线性关系，检出限为8．0×10-9mol／L。结论该方法快速，准确，DNA固定的双层纳米金修饰电极可用于实际样品的测定。Objective To establish the determination method of adenine by glassy carbon electrode modified with double - layer gold nanoparticles （GNPs）, which were anchored with DNA. Methods The electrochemical response of adenine was studied by differential pulse voltammetry （DPV）. Results The double - layer GNPs showed obvious electrocatalytic activity in oxidation of adenine. Under the optimum conditions, the peak currents of adenine were proportional to the concentrations in the range of 3.0 × 10 -8 mol/L - 1.0 × 10 -7 mol/L, 2.0 × 10 7 mol/L -7.0× 10 -7 mol/L with the detection limits of 8.0 × 10 -9 mol/L. Conclusion This method was rapid and accurate, and the glassy carbon electrode modified with DNA - anchored GNPs can be used to determine adenine in the real sample.

关 键 词：DNA 纳米金 玻碳电极 腺嘌呤 

分 类 号：O657.1[理学—分析化学]