葛根素预处理对大鼠局灶性脑缺血再灌注损伤的保护作用及时间窗研究  

Protective effects of puerarin preconditioning on focal cerebral ischemia-reperfusion injury in rats and its therapeutic time window

作　　者：常明则 田晔[1] 乔琳[2] 狄政莉[1] 胡彬[2] 张茹[3] 王虎青[3] 吴海琴[3] 刘勇[4] 

机构地区：[1]陕西省西安市中心医院神经内科,陕西西安710003 [2]延安大学附属医院麻醉科,陕西延安716000 [3]西安交通大学第二医院神经内科,陕西西安710004 [4]西安交通大学医学院神经生物研究所,陕西西安710061

出　　处：《吉林大学学报（医学版）》2014年第1期23-26,I0001,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金面上项目资助课题(81270415);陕西省卫生厅科研基金资助课题(2012D43);陕西省西安市科技局科技计划项目资助课题[SF1319(2)]

摘　　要：目的:观察葛根素(Pur)预处理对局灶性脑缺血再灌注损伤大鼠的干预作用,并探讨其预处理脑保护效应的可能时间窗。方法:40只雄性SD大鼠随机分为缺血再灌注(IR)组及Pur预处理(PC)-6h组、PC-12h组、PC-24h组和PC-48h组,除IR组外,其余4组大鼠分别于脑缺血前6、12、24及48h给予Pur100mg·kg-1腹腔注射行单次预处理,IR组大鼠腹腔注射等容量生理盐水,采用大脑中动脉线栓法阻闭90min再灌注建立局灶性脑缺血再灌注模型,于再灌注后24h行神经功能评分并计算脑梗死体积百分比(BIVP)。结果:与IR组比较,PC-6h、PC-12h及PC-24h组大鼠脑缺血再灌注后24h神经功能评分均明显增加(P<0.01),BIVP明显减少(P<0.01),而PC-6h、PC-12h及PC-24h组间比较差异均无统计学意义(P>0.05)。PC-48h组与IR组大鼠再灌注后24h神经功能评分及BIVP比较差异均无统计学意义(P>0.05)。结论:Pur预处理对大鼠局灶性脑缺血再灌注损伤可产生保护作用,其预处理脑保护效应时间窗可持续达24h。Objective To investigate the interventional effect of puerarin （Pur） preconditioning on focal cerebral ischemia-reperfusion （IR） injury in rats and to explore the possible therapeutic time window. Methods Fourty male Sprague-Dawley rats were randomly divided into IR, Pur preconditioning （PC） -6h, PC-12h, PC-24h and PC-48h groups （n=8）. 100 mg ·kg-1 Pur was given at 6, 12, 24 or 48 h before cerebral ischemia in the rats in last four groups, 0.9% saline 1 ml ·kg-1 was injected ip at the end of 90 min ischemia in the rats in IR group. A focal cerebral ischemia reperfusion model was induced by the middle cerebral artery intraluminal suture method. All animals were subjected to the left middle cerebral artery occlusion for 90 min, followed by reperfusion for 24 h. The neurological deficit score was measured at 24 h after IR. The brain infarct volume percentage （BIVP） was then assessed after 2% TTC staining following the last neurological outcome evaluation. Results Compared with IR group, the neurologic deficit scores in PC-6h, PC-12h and PC-24h groups were statistically improved （P〈0.01）, but not in PC-48h group （P〉0.05）. The BIVP in PC-6h, PC-12h and PC-24h group were significantly decreased compared with IR group （P〈0.01）. No statistically significant difference in BIVP was found between IR group and PC-48h group （P〉0.05）. There were no statistically significant differences in neurologic deficit score and BIVP between PC-6h, PC-12h and PC-24h groups （P〉0.05）. Conclusion Pur precondioning can protect rat brain against IR injury. The therapeutic time window of Pur neuroprotection may be over 24 h.

关 键 词：葛根素 预处理 脑缺血再灌注 神经保护作用 

分 类 号：R285.5[医药卫生—中药学] R743[医药卫生—中医学]