L-氨基胍对体外培养退变椎间盘细胞中炎性因子水平的影响  

Influence of L-aminoguanidine in inflammatory factor levels in degenerate intervertebral disc cells in vitro

作　　者：符俏[1,2] 虞乐华[1] 

机构地区：[1]重庆医科大学附属第二医院康复医学科,重庆404100 [2]海南省人民医院康复医学科,海南海口570311

出　　处：《吉林大学学报（医学版）》2014年第1期35-38,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81171859);重庆市卫生局科研基金资助课题(2010-1-20;2011-2-172)

摘　　要：目的:研究一氧化氮合酶(NOS)抑制剂L-氨基胍(L-AG)对体外培养的退变椎间盘细胞中炎性因子水平的影响,并探讨其作用机制。方法:采用体外培养的退变椎间盘细胞为研究对象,根据L-AG浓度分为0(对照组)、0.5%、1.0%和1.5%组,作用24h后,分光光度法检测NOS活性和一氧化氮(NO)水平,免疫印迹法检测炎性因子白细胞介素1(IL-1)、白细胞介素6(IL-6)以及肿瘤坏死因子α(TNF-α)的表达水平。结果:0.5%、1.0%、1.5%L-AG组退变椎间盘细胞中NOS活性分别为6.1、5.5和4.2U·mL-1,NO水平分别为128.3、116.5和97.7μmol·L-1,与对照组比较均显著降低(P<0.01);与对照组比较,不同浓度L-AG组退变椎间盘细胞中IL-1、IL-6及TNF-α表达水平均显著降低(P<0.01)。结论:NOS抑制剂L-AG可显著降低退变椎间盘细胞炎性反应,其作用机制可能与NOS抑制有关。Objective To study the influence of nitric oxide synthase （NOS） inhibitor L-aminoguanidine （L-AG） in the inflammatory factor levels in degenerate intervertebral disc cells in vitro, and to explore its mechanism. Methods After treatment with L-AG （0, 0.5%, 1.0%, 1.5%） for 24 h, the levels of NO and the activities of NOS were detected with spectrophotometric method and the expression levels of IL-1, IL-6 and TNF-α were determined by Western blotting. Results The activities of NOS in degenerate intervertebral disc cells in 0. 5%, 1.0%, 1.5% L-AG groups were 6.1, 5.5, and 4.2 U · mL-1; the NO levels were 128.3, 116.5, and 97.7 μmol · L-1 , respectively, which were significantIy lower than those in OL-AG group （control group） （P〈 0. 05）. Compared with control group, the expression levels of IL-1, IL-6 and TNF-α in 0.5%, 1.0%, and 1.5% L-AG groups were also significantly reduced （P〈0. 01-0.05）. Oonclusion L-AG can reduce the inflammatory reaction of degenerate intervertebral disc cells, which may be related to inhibition of NOS.

关 键 词：L-氨基胍 退变椎间盘细胞 一氧化氮合酶 炎性因子 

分 类 号：R681.5[医药卫生—骨科学]