再生障碍性贫血患者外周血和骨髓中IL-17、IL-23及TGF-β表达水平及其临床意义  

Expression levels of IL-17,IL-23 and TGF-βin peripheral blood and bone marrow of patients with aplastic anemia and clinical significances

作　　者：李军[1] 黄可欣[2] 陆培信[3] 宋雪松[4] 马洪喜[5] 

机构地区：[1]吉林大学中日联谊医院血液肿瘤科,吉林长春130033 [2]吉林大学基础医学院实验平台中心,吉林长春130021 [3]吉林大学基础医学院生物化学与分子生物学实验中心,吉林长春130021 [4]吉林大学第一医院麻醉科,吉林长春130021 [5]吉林大学第一医院病理科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2014年第1期146-149,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(201115116)

摘　　要：目的:探讨再生障碍性贫血(AA)患者外周血和骨髓中白细胞介素17(IL-17)、白细胞介素23(IL-23)及转化生长因子β(TGF-β)的表达水平及其临床意义,阐明AA的发病机制。方法:随机选取50例AA患者作为实验组,30例同期体检健康人作为对照组;采用酶联免疫吸附法(ELISA)和实时荧光定量PCR法检测外周血、骨髓中IL-17、IL-23、TGF-β蛋白及mRNA表达水平。结果:与对照组比较,实验组患者外周血、骨髓中IL-17和IL-23蛋白及mRNA表达水平明显升高,组间比较差异有统计学意义(P<0.01),其中以IL-17升高最为显著(P<0.001);实验组患者外周血和骨髓中TGF-β蛋白及mRNA表达水平明显降低,组间比较差异亦有统计学意义(P<0.01)。实验组患者外周血和骨髓中IL-17与IL-23蛋白表达水平均呈正相关关系(r=0.69,P<0.05;r=0.71,P<0.05),IL-17与TGF-β蛋白表达水平呈负相关关系(r=-0.65,P<0.05),IL-23与TGF-β蛋白表达水平呈负相关关系(r=-0.72,P<0.05)。结论:IL-17、IL-23和TGF-β可能直接参与AA的发生发展过程,三者的联合检测可为AA患者的临床治疗提供新的思路。Objective To explore the expression levels of IL-17, IL-23 and TGF-β in peripheral blood and bone marrow of the patients with aplastic anemia （AA） and its clinical significances, and to clarify the pathogenesis of AA. Methods 50 cases of AA patients were randomly selected as experiment group and 30 cases of healthy people at the same period were used as control group; Enzyme-linked immunosorbent assay （ELISA） and real-time fluorescence quantitative were performed to detect the expression levels of IL-17, IL-23 and TGF-β proteins and mRNA in peripheral blood and bone marrow. Results Compared with control group, the protein and mRNA levels of IL-17 and IL-23 in peripheral blood and bone marrow of the patients in experiment group were significantly increased, and the differences between two groups were statistically significant （P〈 0.01）, among them, the elevated IL-17 was significant （P〈0. 001） ; the protein and mRNA levels of TGF-13 in peripheral blood and bone marrow of the patients in experiment group were significantly decreased, and the differences between two groups were also statistically significant （P〈0.01）. The protein and mRNA levels of IL-17 and IL-23 in peripheral blood and bone marrow of the patients in experiment group had positive correlation （r=0. 69, P=0. 041; r=0. 71, P〈 0.05）, While the protein levels of IL-17 and TGF-β had negative correlation （r=- 0. 65, P〈0. 05）; and the potein levels of IL-23 and TGF-13 had negative correlation （r= --0.72, P〈0.05）. Conclusion IL-17, IL-23 and TGF-13 may participate in the occurrence and development of AA, and the combined detection of the three indexes provides the new ideas for clinical treatment of AA.

关 键 词：再生障碍性贫血 骨髓 白细胞介素17 白细胞介素23 转化生长因子B 

分 类 号：R556.5[医药卫生—血液循环系统疾病]