分子光谱法研究5-氟尿嘧啶与牛血清白蛋白的相互作用  

Bovine serum albumin interaction with 5-fluorouracil studied using molecular spectroscopy

作　　者：梁彦秋[1] 张宇[1] 

机构地区：[1]沈阳化工大学环境与安全学院,辽宁沈阳110142

出　　处：《化学研究与应用》2014年第1期33-37,共5页Chemical Research and Application

基　　金：辽宁省教育厅项目(20060687)资助;沈阳化工大学博士科研启动项目(200811)资助

摘　　要：采用荧光、紫外和红外光谱法研究了5-氟尿嘧啶(5-FU)同牛血清白蛋白(BSA)的相互作用。5-FU对BSA的内源荧光具有强烈的猝灭作用。二者之间形成不发荧光的复合物是导致荧光猝灭的主要原因。计算了不同温度下两者的结合常数、结合位点数和相应的热力学参数。根据能量转移理论计算了作用距离。采用同步荧光光谱和红外光谱分析了5-FU对BSA构象的影响。The binding of 5-fluorouracil（5-FU）to bovine serum albumin（BSA）was investigated by molecular spectroscopic（fluores-cence,ultraviolet and IR absorption） techniques. 5-FU can strongly quench the intrinsic fluorescence of BSA. The major quenching mechanism was suggested as the formation of nonflurorescence complex between 5-FU and BSA. The binding constants,the number of bonding sites and corresponding thermodynamic parameters at different temperature were calculated. Based on the F？rster’s theory of non-radiation energy transfer,the binding distance was obtained. The effect of 5-FU on the conformation of BSA was analyzed u-sing synchronous fluorescence and IR spectroscopy.

关 键 词：牛血清白蛋白 荧光猝灭 紫外光谱 红外光谱 

分 类 号：O657.3[理学—分析化学]