直链淀粉手性固定相拆分泮托拉唑钠对映体  

Enantiomeric separation of pantoprazole sodium on amylose chiral Stationary phase

作　　者：卢定强[1,2] 王琦[1] 孙生柏 夏芙洁 殷婷婷[1] 凌岫泉[1] 

机构地区：[1]南京工业大学药学院,江苏南京210009 [2]江苏省药物研究所,江苏南京210009

出　　处：《化学研究与应用》2014年第1期65-68,共4页Chemical Research and Application

摘　　要：建立了泮托拉唑钠对映体的高效液相色谱分析方法。在正相条件下,使用Chiralpak AD-H手性色谱柱,考察了流动相中极性调节剂的各个组分及其体积分数、流速以及柱温对泮托拉唑钠对映体拆分的影响。确定了达到最佳拆分效果的条件:流动相为正己烷∶异丙醇(体积比)=60∶40;流速0.6 mL·min-1,检测波长289nm;柱温25℃。研究手性合成了(S)-泮托拉唑钠,对映体过量值(e.e.)达99.53%。在优化的色谱条件下,泮托拉唑钠対映体能达到基线分离且重复性好,可用于其质量控制和手性拆分的研究。A chiral stationary phase HPLC method was established for enantiomeric separation of pantoprazole sodium. By using a chiral column Chiralpak AD-H（250mm＆#215;4. 6mm＆#215;5μm）,the influences of concentration of organic solvent,flow rate and column temperature on the enantiomeric separation were investigated. The most optimal conditions was identified as follows：V（ hexane）∶V （isopropanol）=60∶40;flow rate：0. 6mL.min-1;through wholly wavelength scanning to determine the detection wavelength of Pan-toprazole sodium：289nm;column temperature：25℃. The enantionary excess（e. e. ）of the synthesized（S）-（-）-pantoprazole sodium was 99. 53%. under this optimized chromatographic conditions,the enantiomers of pantoprazole sodium reached a better baseline separation with good repeatability. This conditions could be available to the quality control and chiral separation of pantoprazole so-dium.

关 键 词：(S)-泮托拉唑钠 高效液相色谱法 对映体拆分 

分 类 号：O657.72[理学—分析化学]