拉曼光谱和DSC研究人参皂苷分子与DMPC双层膜的作用  

Raman and DSC Spectroscopic Studies on the Interaction between Ginsenosides and DMPC Bilayer Membranes

作　　者：惠歌[1,2,3] 赵雨[2] 张景洲[2] 刘威[2] 李红东[3] 赵冰[1] 

机构地区：[1]吉林大学超分子结构与材料国家重点实验室,吉林长春130012 [2]长春中医药大学,吉林长春130117 [3]吉林大学超硬材料国家重点实验室,吉林长春130012

出　　处：《光谱学与光谱分析》2014年第2期410-414,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(21273091);高等学校博士学科点专项科研基金项目(20110061110017);国家“重大新药创制”科技重大专项项目(2011ZX09401-305-10);吉林省科技厅重点项目(20110338);吉林省世行贷款农产品质量安全项目(32011-Z43)资助

摘　　要：探讨药物与细胞膜的作用对于改善药物的药理活性、揭示药物的作用机制具有重要意义。利用差示扫描量热(DSC)和拉曼光谱技术对五种人参皂苷分子与DMPC磷脂双层膜的作用进行了实验研究。结果显示,在人参皂苷分子的作用下,DMPC的极性头部骨架构象没有发生变化。Rb1和Rh2增多了脂肪酰链中无序性的构象,增强了侧链排列的无序性,而三醇组皂苷Re,Rf和Rg1对DMPC双层膜的影响较小。DSC结果进一步表明,Rb1和Rh2均与DMPC双层膜发生了强相互作用,两皂苷分子使DMPC的相变温度显著降低,双层膜的流动性增强。Rf对DMPC双层膜的扰动作用要强于Re和Rg1。Study the effect of drugs and biological membranes is of prime importance on understanding drugs ’ curative effects and improving their biological properties .In this article ,Raman spectrum has been combined with differential thermal scanning tech-nology to discuss the relationship between five categories of ginsenoside molecules and DMPC bilayer films .Raman results indi-cated that the saponin molecules have not altered the polarity conformation of O -C-C-N＋ backbone in DM PC bilayers ,and the polarity head still paralleled to the membrane surface .The order of the internal molecular chain and the lateral chain-chain packing have been decreased as the panaxadiol saponins Rb1 and Rh2 increased ,and to the opposite ,the panaxatriol saponins Re ,Rf and Rg1have showed weak effects on DMPC bilayers .The DSC showed further results that the strong effects of ginsen-oside Rb1 and Rh2 on DMPC ,which both have obviously reduced the DMPC molecular phase transition temperature ,thus in-creasing the fluidity of bilayers .In addition ,panaxatriol saponin Rf has displayed stronger disturbance effect on DM PC than Re and Rg1 .

关 键 词：人参皂苷 二醇组 三醇组 拉曼光谱 

分 类 号：O657.3[理学—分析化学]