Mn^(2+)-CTMAB敏化自组装环荧光显微成像技术测定甲苯磺酸妥舒沙星  

Mn^(2+)-CTMAB-Sensitized Fluorescent Microscopic Determination of Tosufloxacin Tosylate Based on a Self-Ordered Ring

作　　者：邓凤玉 黄春秀[1] 刘颖[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081 [2]石药集团有限公司,河北石家庄050035

出　　处：《光谱学与光谱分析》2014年第2期445-449,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(21177163);高等学校学科创新引智计划项目(B08044);中央民族大学"985工程"项目(MUC98504-14;MUC98507-08);中央民族大学"211工程"项目(MUC02121103);中央高校基本科研业务费专项资金项目(0910KYZY45)资助

摘　　要：随着抗菌药物的发展,畜产品中残留的药物对环境及公众健康会构成严重的潜在危害,建立检出限低、选择性好的药物残留检测方法具有非常重要的意义。该工作在pH 10.50的氨-氯化铵缓冲溶液和聚乙烯醇-124介质中,以锰(Mn2+)和十六烷基三甲基溴化胺(CTMAB)为敏化剂,甲苯磺酸妥舒沙星(tosufloxacin tosylate,TSFX)在疏水性玻片表面形成自组装环,建立了检测TSFX的快速方法。当点样体积为0.20μL时,线性范围为4.05×10-14~4.28×10-13 mol·ring-1,检出限为4.10×10-15 mol·ring-1。应用于检测甲苯磺酸妥舒沙星片剂中TSFX含量,测定值(0.144g·片-1)接近于药片的标示值(0.15g·片-1;兔子灌喂TSFX药片后血清中TSFX浓度的加标回收率为90.0%~105.0%,相对标准偏差(RSDs)为1.9%~3.3%。内蒙古锡林郭勒盟是国家重要的畜产品基地,其羊肉是京津唐地区的主要供应源。在羊组织样品(肉、肝脏、肾脏)前处理过程中采用乙腈作萃取剂,萃取液直接用于SOR技术测定,免去样品测定时的过滤步骤。测定结果表明,内蒙古锡盟苏尼特右旗、西乌珠穆沁旗、锡林浩特市、多伦、蓝旗和白旗6个牧场羊组织样品中TSFX加标回收率在92.0%~101.0%,RSDs小于2.7%,结果令人满意。研究成果为相关部门提供了可信的实验数据和理论依据,同时拓宽了SOR技术的应用范围。With the development of antimicrobial drugs ,drug residue in animal products has a serious potential hazards for the environment and public health ,it is urgent to set up drug residue detection method with low detection limit and good selectivity . In our paper ,with poly （vinyl alcohol）-124 （PVA-124） and NH3-NH4Cl （pH 10.50） as the medium ,Mn2＋ and cetyltrimethyl ammonium bromide （CTMAB） as sensitizer ,tosufloxacin tosylate （TSFX） formed a self-ordered ring （SOR） on a hydrophobic glass slide support .When the droplet volume is 0.20 μL ,the TSFX can be detected in the range of 4.05 ＆#215; 10-14 ~4.28 ＆#215; 10-13 mol ＆#183; ring -1 （2.02 ＆#215; 10-7 ~2 .14 ＆#215; 10-6 mol ＆#183; L -1 ） ,and the limit of detection （LOD） can reach 4.1 ＆#215; 10-15 mol ＆#183; ring -1 （2.0 ＆#215; 10-8 mol ＆#183; L -1 ） .The established method had been applied satisfactorily to determine the content of TSFX in tablet ,which close to the marked value of 0.15 g ＆#183; piece-1 ） found value：0.144 g ＆#183; piece-1 ） and the results of TSFX concentrations in rabbit serum at different time after dosing with the recoveries of 90.0% ~105.0% and RSDs 1.9% ~3.3% were satisfactory .Xilinguole of Inner Mongolia is the national important livestock products ground ,whose lamb is the main suppling source for Beijing-Tianjin-Tangshan region .It is very urgent to detect residue of antibacterial drug in its sheep tissue samples （meat ,liver ,kidney） .In this paper ,when acetonitrile was used as extraction agent in pretreatment of sheep tissue samples in six rangelands including Sonid Right Banner ,Xiwuzhumuqin Banner ,Xilinhot City ,Duolun County ,The white Flag Town and The Blue Flag Town ,the extract can be directly determined with SOR technology without filtering process ,the sample standard addition recovery of sheep tissues were 92.0% ~101.0% and RSDs were less than 2.7% .The results indicated that the SOR technique can be successfully applied to pharmaceuticals and biologica

关 键 词：自组装环荧光显微成像技术 甲苯磺酸妥舒沙星 药片 血清 动物组织 内蒙古锡林郭勒盟 

分 类 号：O657.3[理学—分析化学]