Antibody Production for a Rapid Fluorescence Polarization Immunoassay of Estrone  

作　　者：ZHANG Xuan WANG Qiang YU Zhong Sergei A. Eremin YU Chun Fai LIU Jin SUN Yuan Ming LEI Hong Tao 

机构地区：[1]Risk Assessment Key Laboratory of Agricultural Product in Preserving,Ministry of Agriculture/Guangdong Provincial Key Laboratory of Food Quality and Safety,South China Agricultural University [2]Department of Chemistry,M.V. Lomonosov Moscow State University [3]Environmental Science Program,United International College

出　　处：《Biomedical and Environmental Sciences》2014年第1期52-55,共4页生物医学与环境科学（英文版）

基　　金：supported by Natural Science Foundation of China(U1301214);Guangdong Natural Science Foundation(S2013030013338);the PhD Programs Foundation of Ministry of Education of China(20114404130002);National Department Public Benefit Research Foundation(201003008-08)

摘　　要：Estrone has been identified as a potential endocrine-disrupting chemical （EDC）[1]. Estrone is usually quantified by gas chromatography-mass spectrometry （GC-MS）, GC-MS/MS, high performance liquid chromatography （HPLC）, HPLC- MS, and HPLC-MS/MS, etc.[2-3]. Meanwhile, several immunoassays based on radioimmunoassay, enzyme linked immunosorbent assay （ELISA） or chemiluminescence immunoassay （CLIA） for determination of estrone in real samples have been developed[2＇4]. Although these methods are sensitive, they need multistage separation and are thus time-consuming and laborious. A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods （with separation） to homogeneous assays （without separation）[5]. Fluorescence polarization immunoassay （FPIA） is one of the homogeneous techniques that meets the requirements of a simple, reliable, fast, and cost-effective analysis[6]. Therefore, the present study is focused on the development of FPIA in order to analyze estrone based on antibody production.Estrone has been identified as a potential endocrine-disrupting chemical （EDC）[1]. Estrone is usually quantified by gas chromatography-mass spectrometry （GC-MS）, GC-MS/MS, high performance liquid chromatography （HPLC）, HPLC- MS, and HPLC-MS/MS, etc.[2-3]. Meanwhile, several immunoassays based on radioimmunoassay, enzyme linked immunosorbent assay （ELISA） or chemiluminescence immunoassay （CLIA） for determination of estrone in real samples have been developed[2＇4]. Although these methods are sensitive, they need multistage separation and are thus time-consuming and laborious. A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods （with separation） to homogeneous assays （without separation）[5]. Fluorescence polarization immunoassay （FPIA） is one of the homogeneous techniques that meets the requirements of a simple, reliable, fast, and cost-effective analysis[6]. Therefore, the present study is focused on the development of FPIA in order to analyze estrone based on antibody production.

关 键 词：FPIA Antibody Production for a Rapid Fluorescence Polarization Immunoassay of Estrone 

分 类 号：S852.4[农业科学—基础兽医学]