舒肝解郁胶囊的UPLC-MS指纹图谱研究  

UPLC-MS fingerprint of Shugan Jieyu Capsules

作　　者：乔怀耀[1] 罗荣[1] 吴娟[1] 朱文漓[1] 柯尊洪[1] 郝晓锋[1] 柯潇[1] 邬智刚 

机构地区：[1]成都康弘药业集团股份有限公司,四川成都610036

出　　处：《中草药》2014年第2期208-213,共6页Chinese Traditional and Herbal Drugs

基　　金：四川省重大产业技术创新专项"治疗抑郁症中药新药舒肝解郁胶囊产业化工艺研究"(2012CD00082);四川省科技成果转化项目"治疗抑郁症中药新药舒肝解郁胶囊成果转化"(2012SC0029)

摘　　要：目的建立舒肝解郁胶囊的UPLC-MS指纹图谱分析方法,为其质量评价积累数据。方法以0.1%甲酸-乙腈为流动相,梯度洗脱60 min,使用Agilent Poroshell 120 SB-C18色谱柱(150 mm×4.6 mm,2.7μm)进行指纹图谱研究,同时通过高效液相色谱-电喷雾离子阱质谱分析方法鉴定部分成分。结果对其中22个共有峰进行了成分鉴定,对34批舒肝解郁胶囊进行了指纹图谱检测和相似度评价,34批样品的相似度均大于0.960,确立了指纹图谱中的12个特征吸收峰。结论所建立的舒肝解郁胶囊的指纹图谱特征性和专属性强,该方法可用于舒肝解郁胶囊的质量控制。Objective To establish aultra high performance liquid chromatography coupled with mass spectrometry （UPLC-MS） fingerprint for the quality control of Shugan Jieyu Capsules and to accumulate the data used for their quality evaluation. Methods UPLC separation was performed on an Agilent Poroshell 120 SB-Cls column （150 mm× 4.6 mm, 2.7 μm） by gradient elution in 60 min with 0.1% formic acid-acetonitrile as mobile phase. The mass spectrometry detection was performed on a trap equipped with an ESI interface and operated in both positive and negative ionization modes to identify components. Results Twenty-two characteristic common peaks of chemical constituents were identified and 34 batches of Shugan Jieyu Capsules were analyzed. The quanlity of samples was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM （2004 A version）. The similarities of 34 batches of Shugan Jieyu Capsules were all over 0.960, and 12 characteristic peaks of UPLC fingerprint for Shugan Jieyu Capsules were established. Conclusion The UPLC fingerprint of Shugan Jieyu Capsules is established, the convenient and high specific method could be used to identify and evaluate the quality of Shugan Jieyu Capsules.

关 键 词：舒肝解郁胶囊 指纹图谱 UPLC-MS 相似度 质量评价 绿原酸 紫丁香苷 咖啡酰奎宁酸 芦丁 金丝桃苷 槲皮素 Ⅱ3 Ⅱ8穗花杉双黄酮 

分 类 号：R286.02[医药卫生—中药学]