刺五加有效成分提取工艺考察及不同产地刺五加中有效成分量的比较  

Extraction technology for active constituents in Acanthopanax senticosus and comparison on its contents of A.senticosus from various habitats

作　　者：樊如强 傅宏征[2] 金学英[1] 王建斌[2] 高纯[2] 盖春燕[2] 胡荣[1] 

机构地区：[1]扬州大学医学院药学系,江苏扬州225009 [2]北京大学医学部天然药物及仿生药物国家重点实验室,北京100191

出　　处：《中草药》2014年第2期260-264,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81172943)

摘　　要：目的研究刺五加有效成分的提取工艺及比较不同产地刺五加中有效成分的量。方法采用HPLC法测定刺五加中紫丁香苷、刺五加苷E和异嗪皮啶。确定提取溶媒,采用正交试验法考察加溶媒量、提取时间和提取次数对提取工艺的影响,确定最佳提取工艺。根据最佳提取工艺,比较不同产地刺五加中紫丁香苷、刺五加苷E和异嗪皮啶的量。结果正交试验所得的最佳提取条件为加10倍量的50%乙醇,提取3次,每次2 h。黑龙江产的刺五加所含紫丁香苷、刺五加苷E和异嗪皮啶量较高。结论该提取工艺合理、稳定、可行;不同产地刺五加药材中紫丁香苷、刺五加苷E和异嗪皮啶的量存在一定的差异。Objective To study the extraction technology for the active constituents from Acanthopanax senticosus and to compare their contents from various habitats. Methods The contents of syringin, eleutheroside E, and isofraxidin in A. senticosus were measured using HPLC method. The extracting menstrua were selected, and the influence of the menstruum dosage, extracting times, and extracting time were investigated using orthogonal design method. Based on the optimal technology, the contents of syringin, eleutheroside E, and isofraxidin in A. senticosu from various habitats were compared. Results The optimal conditions for the extraction technology were as follows： extracting for three times, each time for 2 h with 10 times the amount of 50% ethanol. A. senticosus produced in Heilongjiang province had higher contents of syringin, eleutheroside E, and isofraxidin. Conclusion This extraction technology is reasonable, stable, and feasible. The contents of syringin, eleutheroside E, and isofraxidin in A. senticosus from different habitats have some differences. The multi-index contents of syringin, eleutheroside E, and isofraxidin could reflect the quality ofA. senticosus more comprehensively.

关 键 词：刺五加 紫丁香苷 刺五加苷E 异嗪皮啶 正交试验 

分 类 号：R282.4[医药卫生—中药学]