栀子大黄汤UFLC指纹图谱研究  

Fingerprint of Zhizi Dahuang Decoction

作　　者：唐峥[1] 毕开顺[2] 韩飞[2] 朱鹤云[2] 陈科霖 王粉绒 尹然[2] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中草药》2014年第3期367-372,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金青年科学基金项目(81202893);辽宁省教育厅科学研究一般项目(L2012360)

摘　　要：目的建立栀子大黄汤(Zhizi Dahuang Decoction,ZDD)的超快速液相色谱(UFLC)指纹图谱,并研究全方与其组成药味的相关性。方法采用RP-UFLC法,以Shimadzu Shim-pack XR-ODS色谱柱(75 mm×3.0 mm,2.2μm)为分析柱,乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,体积流量为1.0 mL/min,检测波长为254 nm。结果建立ZDD的UFLC指纹图谱,标定51个共有色谱峰,并通过对照品比对指认了其中的14个色谱峰;考察ZDD全方与各组成药味指纹图谱的相关性,对各共有色谱峰进行了峰位归属。结论所建立的UFLC指纹图谱分析方法快速、可靠,重复性好,可为ZDD物质基础和质量控制方法的研究提供参考。Objective To establish the ultra-fast liquid chromatographic （UFLC） fingerprint ofZhizi Dahuang Decoction （ZDD）, and to study the correlation between ZDD and its composition herbs. Methods The separation was achieved on a Shimadzu Shim-pack XR-ODS column （75 mm × 3.0 mm, 2.2 um ） by gradient elution with acetonitrile-0.1% formic acid solution as the mobile phase. The flow rate was 1.0 mL/min and the measurement wavelength was 254 nm. Results The UFLC fingerprint of ZDD was established and 51 characteristic common peaks were found, among which 14 peaks were recognized by comparing with reference substances. Furthermore, the peak attribution was analyzed so as to investigate the correlation between the fingerprint of composition herbs and ZDD. Conclusion The method is rapid, accurate, reliable, and reproducible. The established fingerprint could provide the references for the study of substance basis and quality control of ZDD.

关 键 词：栀子大黄汤 指纹图谱 相关性 超快速液相色谱 质量控制 

分 类 号：R286.02[医药卫生—中药学]