CaKR1上游启动子分离及其表达分析  

Isolation and Expressional Analysis of Upstream Promoter of Gene CaKR1

作　　者：黄雪盈[1,2] 石兰平[1,2] 杨晟[1,2] 卢蓉[1,2] 王博[1,2] 申磊[1,3] 刘艳艳[1,3] 洪水秀 梁浩[1,3] 刘志钦[1,2] 贺俐[4] 吴杨[4] 何水林[1,3] 

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室,福建福州350002 [2]福建农林大学生命科学学院,福建福州350002 [3]福建农林大学作物科学学院,福建福州350002 [4]井冈山大学生命科学学院,江西吉安343009

出　　处：《江西农业学报》2014年第2期49-54,共6页Acta Agriculturae Jiangxi

摘　　要：运用基因组步移技术分离获得了CaKR1基因上游-1594 bp的启动子序列,命名为CaKR1p,发现其中含有SA、ABA、低温等信号应答原件以及其它诸如W盒等应答逆境胁迫的调控原件。进一步构建CaKR1p与GUS报告基因融合的植物表达载体,获得了烟草转基因植株及其相应的T1代株系,利用T1代转基因株系分析了CaKR1p在几种外源激素处理下的GUS基因的表达,结果表明外源激素SA、JA和ABA的诱导处理均可激活该启动子下游报告基因GUS的表达,说明CaKR1的表达和作用受到SA、ABA以及JA等信号通路调节。In the present study , the -1594 bp upstream promoter sequence of gene CaKR1 was isolated by using genome walk-ing technology and named CaKR1p, and cis-elements such as SARE, ABRE, LTRE were found in this promoter.The promoter was fused to GUS reporter gene to generate plant expression vector .This expression vector was transformed into tobacco plants by u-sing Agrobacterium-mediated method , and the transgenic tobacco plants were acquired and confirmed .With T1 transgenic tobacco lines, we analyzed the expression of CaKR1p against the treatments of several exogenous hormones , and found that the expression of GUS reporter gene was induced significantly by exogenously -applied ABA, JA and SA, suggesting that the expression and function of CaKR1 are regulated by the signal paths of SA , ABA, JA and so on.

关 键 词：辣椒 烟草 锚定蛋白 启动子 表达 转基因 

分 类 号：Q943.2[生物学—植物学]