凉血通瘀方对实验性瘀热证脑缺血大鼠的治疗作用  

Therapeutic Effect of Liangxue Tongyu Formula on Experimental Mixed Stasis-Heat Syndrome of Cerebral Ischemia in Rats

作　　者：蒋宝平[1,2,3] 田磊 李申[5] 许立[1,2] 方泰惠[1,2] 程海波 

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏省中药药效与安全性评价重点实验室,江苏南京210023 [3]江苏省名医验方研究与产业化工程实验室,江苏南京210023 [4]南京中医药大学附属医院,江苏南京210029 [5]河南护理职业学院,河南安阳455000

出　　处：《中药新药与临床药理》2014年第1期10-14,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金青年基金(81102885);江苏省中医药局科技项目(LZ11194);江苏省自然科学基金(BK2007242);国家973计划(2006CB504807)

摘　　要：目的研究凉血通瘀方对实验性瘀热证脑缺血大鼠的治疗作用。方法大鼠注射大肠杆菌内毒素后,采用插线阻断大鼠中动脉法制备瘀热证脑缺血模型,观察凉血通瘀方对模型大鼠的行为学评分,脑梗死率,血液流变学指标,血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6),脑组织中一氧化氮(NO)、一氧化氮合酶(NOS)等指标的影响。结果凉血通瘀方能减轻脑缺血损伤,改善血液流变学指标,降低模型大鼠血清中IL-1β、IL-6含量,抑制脑组织中NOS活性,降低NO含量。结论凉血通瘀方能减轻瘀热证脑缺血模型大鼠的脑缺血损伤,同时能改善"瘀"、"热"症状。Objective To investigate the therapeutic effect of Liangxue Tongyu Formula on experimental mixed stasis-heat syndrome of cerebral ischemia in rats. Methods The model was established by middle cerebral artery occlusion （MCAO） after injection with lipopolysaceharide（LPS）. The effect of Liangxue Tongyu Formula on behavior grading, ratio of cerebral ischemia, hemorrheologie indexes, serum IL-I[3 and IL-6 contents, cerebral NO content and NOS activity were evaluated after administration. Results Liangxue Tongyu Formula on significantly reduced the injury of cerebral ischemia, ameliorated hemorrheologic indexes, decreased the serum IL-I[3 and IL-6 contents, inhibited the cerebral NOS activity and decreased cerebral NO content. Conclusion Liangxue Tongyu Formula not only alleviates the injury of cerebral ischemia but also ameliorate the symptoms of blood stasis and heat syndromes in cerebral ischemia rats with mixed stasis-heat syndrome.

关 键 词：凉血通瘀方 瘀热证 脑缺血 血液流变学 

分 类 号：R285.5[医药卫生—中药学]