香鳞毛蕨有效部位对申克孢子丝菌酵母相的体外抗真菌作用研究  

In Vitro Antifungal Activity of Active Fraction of Dryopteris fragrans Against Sporothrix schenckii Complex in Yeast Phase

作　　者：万江帆[1] 沈志滨[1] 江涛[1,2] 唐春萍[1] 王洁[1] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]广东药学院动物实验中心,广东广州510006

出　　处：《中药新药与临床药理》2014年第1期44-48,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：科技部"十二五"重大新药创制(2011ZX09102-007-03);广东省科技厅科技计划项目(2010B030700044;2011A030100013);2010年广东省教育厅高层人才资助项目;广州市科技局科技计划项目(12A062131631)

摘　　要：目的探讨香鳞毛蕨有效部位对申克孢子丝菌的体外抑菌作用,进一步明确香鳞毛蕨有效部位的抗菌谱。方法取4株申克孢子丝菌为受试菌株,培养至酵母相,分别采用琼脂扩散法测定抑菌圈直径和微量稀释法测定最低抑菌浓度(MIC)与最低杀菌浓度(MFC)。结果香鳞毛蕨有效部位对4株申克孢子菌酵母相的抑菌圈平均直径为25.5 mm,对4株申克孢子丝菌酵母相的MIC几何均数为生药0.18 mg·mL-1,但MFC>生药20mg·mL-1。结论香鳞毛蕨有效部位对申克孢子丝菌酵母相有较强抑菌作用,但无杀菌作用。Objective To determine antifungal activity of active fraction of Dryopteris fragrans against Sporothrix schenckii Yeast Phase in vitro, and further to clarify it＇s antimicrobial spectrum. Methods Four isolates of Sporothrix schenckii were cultured and developed into yeast phase, and then agar diffusion test and microdilution assay were used to obtain the minimum inhibitory concentration （MIC） and minimum fungicide concentration（MFC） value of the four isolates. Results Mean diameter of inhibition zones of 4 isolates of S. schenckii was 25.5 mm, while their geometric mean of MIC was 0.18 mg. mL^-1 （for the crude herb） and that of MFC was over 20 mg. mL^-1（for the crude herb）. Conclusion Active fraction of Dryopteris fragrans exerts strong inhibitive effect against S. schenckii, but has no antifungal action in vitro.

关 键 词：申克孢子丝菌 香鳞毛蕨 体外抗真菌作用 

分 类 号：R285.5[医药卫生—中药学]