HPLC-MS/MS法测定柴葛解肌汤中柴胡皂苷a、b2的含量  

Determination of Saikosaponin a and Saikosaponin b2 in Chaige Jieji Decoction by HPLC-MS/MS

作　　者：谢友良[1] 毕何锋 张婕[1] 蒋东旭[1,2] 赖小平[1,2] 杨俊标 高华裕 

机构地区：[1]广州中医药大学,广东广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808 [3]广东德鑫制药有限公司,广东江门529100

出　　处：《中药新药与临床药理》2014年第1期71-74,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省高新区发展引导项目(2011B010600045);省部产学研结合科技创新平台(201213090600007)

摘　　要：目的建立测定柴葛解肌汤中I型柴胡皂苷a与Ⅱ型柴胡皂苷b2的HPLC—MS／MS方法。方法采用Hypersil GOLDaQ色谱柱（150mm×2．1mm，3txm），乙腈-0．1％甲酸溶液为流动相，流速为300μL.min^-1，柱温：25℃，以液相色谱分离、电喷雾离子化串联进行检测。结果柴胡皂苷a、b2分别在44．8-672ng·mL～、47．2～708ng·mL。线性关系良好（r=0．9992、r=0．9993），平均回收率分别为98．53％和99．47％，RSD分别为2．01％和2．08％（n=6）。结论该方法快速简便、准确、灵敏度高，可用于柴葛解肌汤中柴胡皂苷a、b2的含量测定。Objective To establish an HPLC-MS /MS method for the determination of saikosaponin a and saikosaponin b2 in Chaige Jieji decoction. Methods The separation was performed by using HPLC method with Hypersil GOLD aQ （150 mm ×2.1 mm, 3 μm） as chromatographic column. The mobile phase consisted of acetonitrile -0.1% formic acid and the flow rate was 300 μL.min^-1, the column temperature was set at 25 ℃, and the detection was done by electrospray ionization mass spectrometry. Results The linear range was 44.8-672 ng.mL^-1 for saikosaponin a（r=0.9992）, and 47.2-708 ng.mL^-1 for saikosaponin b2（r=0.9993）. The average recovery rates of saikosaponin a and saikosaponin b2 were 98.53 %, 99.47 %, and RSD were 2.01%, 2.08 %（n=6） , respectively. Conclusion The established method is simple, quick, sensitive and accurate, can be used to determine the content of saikosaponin a and saikosaponin b2 in Chaige Jieji decoction.

关 键 词：柴葛解肌汤 柴胡皂苷A 柴胡皂苷b2 HPLC-MS MS 

分 类 号：R284.1[医药卫生—中药学]