乙醇诱导人胃上皮细胞株凋亡的研究  

Establishment of Human Gastric Epithelial Cells Apoptosis Model Induced by Ethanol

作　　者：刘翠玲[1] 黄可儿[2] 陈柏书[1] 来慧丽[1] 

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学第一附属医院,广东广州510405

出　　处：《中药新药与临床药理》2014年第1期101-106,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81072946)

摘　　要：目的探讨建立乙醇诱导人胃上皮细胞株(GES-1)凋亡模型的方法和可行性。方法采用罗氏细胞实时动态分析仪、MTT比色法、Hoechst染色法、Annexin V-FITC/PI凋亡流式检测研究不同浓度的乙醇对人胃上皮细胞株(GES-1)凋亡作用。结果 (1)当乙醇浓度大于0.5 mol·L-1时,对GES-1细胞株有明显的诱导凋亡作用,而考察的TNF-α(100,50 ng·mL-1)则没有明显的细胞凋亡诱导作用;(2)通过形态学观察,发现乙醇模型组GES-1细胞株出现明显的凋亡细胞形态学改变;(3)Annexin V-FITC/PI凋亡流式分析结果发现乙醇浓度大于0.2 mol·L-1对细胞具有明显的诱导凋亡作用,乙醇主要诱导GES-1细胞株进入早期凋亡,且具有浓度依赖性,但该诱导作用具有时效性,在作用持续60 min后,其诱导作用会逐渐减弱。结论乙醇对GES-1细胞株具有明显的诱导细胞凋亡作用,且具有浓度依赖性及时效性;本凋亡模型具有较好的重现性。Objective To explore the feasibility of establishing the model of human gastric epithelial cells（GES-1 ） apoptosis model induced by ethanol. Methods Cell viability and apoptosis induced by ethanol were assessed by MTr assay, cell real-time dynamic analyzer, Hoechst staining, and Annexin V-FITC/PI. Results （1）When the concentration of ethanol in cell culture medium was higher than 0.5 tool .L^-1, GES-1 showed obvious injury and apoptosis. TNF-α （100, 50 ng.mL^-1） showed no obvious apoptosis induction for the GES-1. （2）The morphological examination showed that the GES-1 of ethanol group appeared apoptotic morphological changes. （3）The results of Annexin V - FITC/PI combined with flow cytometry analysis showed that ethanol at the concentration higher than 0.2 mol＇L-~ induced the apoptosis of GES-1, and the apoptotic cells were almost in early-stage apoptosis, the effect being concentration-dependent. But after 60 minutes, the effect would gradually weaken. Conclusion The results demonstrated that ethanol can induce the apoptosis of GES-1 in time- and dose-dependent. The established apoptosis model has good reproducibility.

关 键 词：人胃上皮细胞 乙醇 凋亡 

分 类 号：R285.5[医药卫生—中药学]