血小板-白细胞聚集体在全麻手术患者中的变化观察  

Platelet-leukocyte aggregates in patients with surgery under general anesthesia

作　　者：王升[1] 李新刚[1] 赵继全[1] 

机构地区：[1]安阳地区医院血液实验室,河南455000

出　　处：《中国临床新医学》2014年第1期33-36,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

摘　　要：目的观察全麻手术患者术后血小板-白细胞聚集体(PLA)的变化,探讨全麻手术患者深部静脉栓塞发生的机理。方法对112例全麻手术患者分别于麻醉诱导前、手术结束后、术后3 d采集标本,利用流式术检测血小板-粒细胞聚集体(PNA)和血小板-单核细胞聚集体(PMA),观察PNA和PMA的变化。结果 112例全麻手术患者麻醉诱导前PNA为(19.32±3.48)%,PMA为(26.86±5.05)%;手术结束后PNA为(19.48±3.20)%,PMA为(31.86±5.31)%;术后3d PNA为(21.85±2.84)%,PMA为(77.69±5.72)%。麻醉诱导前的PNA和PMA与手术结束后、术后3 d比较差异均有统计学意义(P<0.01)。结论全麻手术对患者PNA和PMA均有影响,尤其对PMA影响明显,因此PNA和PMA可以作为判断血小板活化程度和预防术后栓塞的敏感性观察指标。Objective To monitor the platelet-leukocyte aggregates （ PLA） level in patients received surgery under general anesthesia and to explore the potential mechanism of deep vein thrombosis occurred in these patients . Methods Blood samples were collected from 112 patients received surgery at the time：before the general anesthesia;immediately after the surgery , and 3 days after the surgery .Flow cytometry was used to measure the platelet-neutro-phil aggregates （ PNA ） and platelet-monocyte aggregates （ PMA ） .Results Before the general anesthesia： PNA （19.32 ±3.48）%, PMA（26.86 ±5.05）%;Immediately after the surgery：PNA（19.48 ±3.20）%, PMA（31.86 ± 5.31）%;Three days after the surgery：PNA（21.85 ±2.84）%, PMA（77.69 ±5.72）%; Statistical analysis： the PNA and PMA level in patients immediately after the surgery and that in patients three days after surgery were higher than that patients before the general anesthesia （ P〈0.01 ） .Conclusion The PNA and PMA level , especially , the PMA level in patients received surgery is affected by general anesthesia .PNA and PMA can be used as a sensitive bi-omarker to determine the degree of platelet activation and to predict the possible postoperative thrombosis .

关 键 词：血小板 白细胞聚集体 全麻手术 流式细胞术 

分 类 号：R614.2[医药卫生—麻醉学]