儿童烧伤患者感染MRSA的耐药性及分子流行病学研究  

The resistance and molecular epidemiology analysis of MRSA isolated from clinical samples of burn injured children

作　　者：陈华桂[1,2] 周秀萍[2] 

机构地区：[1]怀化市第三人民医院检验科 [2]怀化医专检验系

出　　处：《国际检验医学杂志》2014年第3期299-300,303,共3页International Journal of Laboratory Medicine

摘　　要：目的调查儿童烧伤患者耐甲氧西林金黄色葡萄球菌(MRSA)的耐药性及分子流行病学情况,为控制感染提供科学依据。方法通过PCR扩增mecA基因鉴定MRSA菌株,K-B纸片法检测菌株耐药性,随机扩增多态性DNA(RAPD)对其进行同源性分析。结果从住院烧伤儿童临床标本分离得到的52株金黄色葡萄球菌中MRSA占44.2%(23/52),且对多种抗菌药物均耐药。23株MRSA经RAPD分析可分为4型,以Ⅱ型为主。结论儿童烧伤患者分离MRSA菌株具有多重耐药性,应根据药敏试验结果合理选用抗菌药物进行治疗;RAPD技术能为控制感染提供分子流行病学依据。Objective To investigate the status of antibiotics resistance and the genetic homology of Methicillin-resistant Staph- ylococcus aureus（MRSA） isolated from clinical samples of burn injured children and provide scientific basis for MRSA infection control. Methods MRSA strains was identified by PCR of mecA. Drug resistance was detected by using K-B method. The random amplified polymorphic DNA （RAPD） technique was used to identify the genotypes. Results MRSA strains accounted for 44.2%（23/52） in the Staphylococcus aureus isolated from clinical samples of children suffering from burn injury. All 23 stains MRSA produced fingerprints can be classified into four genotypes,of which type II was the dominanted genotype. Conclusion The MRSA isolated from clinical samples of children suffering from burn injury has multi-drug resistance,and antibiotics should be chosen ac- cording to drug susceptibility test results.

关 键 词：耐甲氧西林金黄色葡萄球菌 耐药性 随机扩增多态DNA技术 烧伤 儿童 

分 类 号：R726.2[医药卫生—儿科]