花生AhSOS2基因原核表达载体的构建及表达  

Construction and Expression of Prokaryotic Expression Vector of AhSOS2 Gene from Peanut

作　　者：张国嘉[1,2] 郑世刚[1] 刘晓[1] 李臻[1] 王庆国[1] 戴绍军[2] 刘炜[1] 

机构地区：[1]山东省农业科学院生物技术研究中心/山东省作物遗传改良与生态生理重点实验室,山东济南250100 [2]东北林业大学盐碱地生物资源环境研究中心/东北油田盐碱植被恢复与重建教育部重点实验室,黑龙江哈尔滨150040

出　　处：《山东农业科学》2014年第1期6-9,共4页Shandong Agricultural Sciences

基　　金：国家转基因生物新品种培育重大专项(2009Z×08001-010B);山东省农业良种工程项目"农业生物资源创新利用研究"(2012-2014)

摘　　要：SOS2(Salt Overly Sensitive 2)是植物中一类耐盐相关基因的统称,在植物对盐害的响应及适应过程中具有重要作用。本试验以花生AhSOS2基因全长cDNA为模板,经PCR扩增获得AhSOS2的蛋白编码区,将该区段与表达载体pET-28a(+)融合,构建获得原核表达载体pET-28a-AhSOS2,并进一步在大肠杆菌BL21(DE3)中进行表达。经1.0 mmol/L IPTG诱导后,获得一条约51 kD的融合蛋白条带,且随着IPTG诱导时间的延长,蛋白表达量逐渐提高,诱导8 h可获得较高的蛋白表达水平。SOS2 （ Salt Overly Sensitive 2 ） is a kind of salt-tolerant related genes in plant .It plays an im-portant role in the response and adaptation process of plants to salt damage .Using the full-length cDNA of AhSOS2 gene as template , its coding region was amplified by PCR method .The recombined prokaryotic ex-pression vector pET-28a-AhSOS2 was constructed successfully by fusing AhSOS2 with expression vector pET -28a（＋）.Then the recombinant was expressed in E.coli strain BL21（DE3）.Inducing with 1.0 mmol/L IPTG, a 51 kD fusion protein was obtained .Its expression quantity improved gradually with the extension of induction time, and attained the highest level after inducing for 8 hours.

关 键 词：花生 AhSOS2 原核表达 融合蛋白 AhSOS2 

分 类 号：Q788[生物学—分子生物学]