麻核桃遗传多样性的SSR分析  

Genetic Diversity Analysis of Juglans hopeiensis Hu by SSR Markers

作　　者：贾建云[1] 陈新[1] 刘洪对[2] 徐丽[1] 张力思[1] 刘庆忠[1] 

机构地区：[1]山东省果树研究所/山东省果树生物技术育种重点实验室,山东泰安271000 [2]山东省农业科学院,山东济南250100

出　　处：《山东农业科学》2014年第1期15-18,共4页Shandong Agricultural Sciences

基　　金：科技部科技基础性工作资助项目"国家核桃板栗种质资源平台(2013-048)";农业部作物种质资源保护项目(2014NWB009);山东省农业良种工程-鲁科农字(2011-086)

摘　　要：本试验利用SSR标记技术对20个麻核桃品种的遗传多样性进行研究。从12对引物中筛选出6对用于正式PCR扩增，共检测到52个遗传位点，其中多态性遗传位点50个，多态位点百分率为96％。遗传相似性分析结果显示麻核桃种质间具有丰富的遗传多样性，遗传相似系数变幅为0．17～0．90，说明SSR标记能将20份种质完全区分开。 UPGMA聚类结果显示，供试材料在相似系数为0．61处聚为4类。The genetic diversity of 20 Juglans hopeiensis Hu varieties was analyzed by simple sequence repeat （ SSR） markers.Six pairs of primers were selected for formal PCR amplification .Fifty-two genetic lo-ci including 50 polymorphic loci were detected with the polymorphic locus percentage as 96%.Genetic simi-larity analysis showed that there were abundant genetic diversities among different walnut varieties .The genetic similarity coefficients ranged from 0.17 to 0.90, which indicated that the 20 walnut varieties could be com-pletely separated by these SSR markers .UPGMA cluster results showed that they were divided into 4 catego-ries when the similarity coefficient was 0.61.

关 键 词：麻核桃 遗传多样性 

分 类 号：Q754[生物学—分子生物学]