不同煎煮方式对柴芍肠宁胶囊中盐酸小檗碱含量的影响  

Effects of Different Decoction Ways on Content of Berberine in Chaishao Changning Capsules

作　　者：刘耀[1] 张永萍[1] 唐云[1] 王万莲 徐剑[1] 胡文言 叶祖光[3] 

机构地区：[1]贵阳中医学院,贵阳550002 [2]北京天地外医药科技有限公司,北京100086 [3]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2014年第4期19-21,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重大"新药创制"国家科技重大专项(2012ZX09101);第二批国家级大学生创新创业训练计划项目(201210662002);黔教高发([2012]426号)

摘　　要：目的：建立柴芍肠宁胶囊中盐酸小檗碱的含量测定方法，考察不同煎煮方式对盐酸小檗碱含量的影响。方法：采用HPLC测定盐酸小檗碱含量，DIKMAODS—C18色谱柱（4．6mm×250mm，5μm），流动相乙腈．0．1％磷酸（每100mL加十二烷基磺酸钠0．1g）（50：50），检测波长345nm。考察黄连与其他药味合煎或分煎时对盐酸小檗碱含量的影响。结果：盐酸小檗碱在2．4～24mg·L-1线性关系良好（r=1），平均回收率101．14％，RSD2．48％。分煎、合煎样品中盐酸小檗碱平均质量分数分别为0．1282，0．0786mg·g，RSD依次为1．02％，1．25％。结论：黄连与其他药味分煎时盐酸小檗碱含量显著高于合煎，为柴芍肠宁胶囊的临床应用提供参考。Objective： To establish a method for determining the content of berberine in Chaishao Changning capsules and investigate effects of different decoction ways on the content of berberine. Method ： HPLC was adopted to determine the content of berberine with DIKMA ODS-C18 column （4.6 mm ×250 mm, 5 μm） , mobile phase of acetonitrile-0.1% phosphoric acid （per 100 mL plus sodium dodecyl sulfate 0. 1 g） （50： 50） and detection wavelength at 345 nm. Effects of mixed decoction and single decoction of Coptidis Rhizoma with other herbs on the content of berberine were investigated. Result： Berberine had a goog linear relationship with 2.4-24 mg -L-1 with r = 1, the average recovery was 101.14% with RSD of 2.48%. Average contents of berberine from decoction and separate decoction samples were 0. 128 2, 0. 078 6 mg.g-1 with RSD of 1.02% , 1.25% , respectively. Conclusion： The content of berberine from mixed decoction samples was significantly higher single decoction samples, but it should be made conclusion through clinical trials.

关 键 词：柴芍肠宁胶囊 合煎 分煎 盐酸小檗碱 黄连 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]