复方颐神养脑胶囊对多发性脑梗死痴呆大鼠的影响  

Influence of Compound Yishen Yangnao Capsule on Multi-infarct Dementia Rats

作　　者：周青罡 李俊[2,3] 舒心莹 朱伟[2] 陈筱[2] 陈兰英[2,3] 

机构地区：[1]江西汇仁药业有限公司,南昌330000 [2]中药固体制剂制造技术国家工程研究中心,南昌330006 [3]江西中医药大学,南昌330006

出　　处：《中国实验方剂学杂志》2014年第4期107-110,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项:重大新药创制(2011ZX09101-003-02)

摘　　要：目的:观察中药复方颐神养脑胶囊(YSYN)对大鼠多发性脑梗死痴呆(MID)的疗效及作用机制。方法:3月龄SD大鼠90只,于左侧颈外动脉注入复合血栓诱导剂,复制多发性脑梗死痴呆模型,动物随机分为模型组、YSYN高剂量组(960 mg·kg-1)、YSYN中剂量组(480 mg·kg-1)、YSYN低剂量组(240 mg·kg-1)、西药阳性组(易倍申)(0.5 mg·kg-1)以及YSYN合西药阳性药组(480 mg·kg-1+0.5 mg·kg-1),另设10只动物为假手术组,造模后第3天,各组均以10 mL·kg-1灌胃给药,1次/d,连续4周,模型组和假手术组给予等量蒸馏水。采用Morris水迷宫法进行学习记忆测试;采用酶联免疫法测定海马组织去甲肾上腺素(NE)水平;采用比色法测定海马组织乙酰胆碱酯酶(AchE)、乙酰胆碱转移酶(CHAT)和谷胱甘肽过氧化物酶(GSH-Px)水平。结果:与假手术组比较,模型组大鼠学习记忆能力显著降低(P<0.05,P<0.01),海马组织AchE含量显著升高(P<0.05),海马组织CHAT、NE以及GSH-Px含量显著降低(P<0.01);与模型组比较,YSYN能够显著提高多发性脑梗死痴呆大鼠学习记忆能力(P<0.05);显著降低多发性脑梗死痴呆大鼠海马组织AchE含量,提高多发性脑梗死痴呆大鼠海马组织CHAT、NE及GSH-Px含量(P<0.05,P<0.01)。结论:YSYN对MID具有良好的保护作用,其作用机制可能是通过调节海马组织内去甲肾上腺素、乙酰胆碱及谷胱甘肽的活性来实现的。Objective： To investigate the effect of compound Yishen Yangnao capsule （YSYN） on multi- infarct dementia （MID） rats and its mechanism. Method： The multi-infarct dementia model, were induced by injecting compound thrombosis inducer into the external carotid artery. Sixty SD rats were randomly divided into model group, YSYN high dose group （960 mg .kg-1 ） , middle dose group （480 mg .kg-1 ） , low dose group 240 mg . kg-1, western medicine positive group （Ebixa, 0.5 mg .kg-1） , YSYN ＋ Ebixa group （480 mg -kg-1 ＋ 0.5 mg. kg-1）, sham operation group. From 3 days after modeling, corresponding drugs were given once daily, lasting four weeks. The Morris water maze was used to test the abilities of learning-memory, enzyme-linked immunsorbent was used to assay the levels of norepinephrine （NE） of hippocampal tissue, and chemocolorimetry was used to assay the levels of acetylcholinesterase （AchE）, cholinacetyhrans-ferase （CHAT）, glutathione peroxidase （GSH-Px）. Result： Compared with sham operation group, learning memory abilities of model group rats were significantly decreased （P 〈 0.05, P 〈 0.01 ） , the content of AchE significantly rose （P 〈 0.05 ） and CHAT and GSH-Px significantly decreased. Compared with model group, the YSYN could significantly improve the abilities of learning memory in MID rats （P 〈 0.05） ; decreased the level of Ache increased the level of CHAT, NE and GSH-Px （P 〈 0.05, P 〈 0.01 ）. Conclusion： The YSYN could significantly improve the abilities of learning memory in MID rats, and has a good protective effect on MID rats. Its mechanism may adjust the level of NE, AchE, CHAT and GSH-Px of hippocampus tissue.

关 键 词：多发性脑梗死痴呆 颐神养脑胶囊 乙酰胆碱 谷胱甘肽过氧化物酶 去甲肾上腺素 

分 类 号：R285.5[医药卫生—中药学]