酸枣仁总皂苷对失眠老年大鼠脑氨基酸类神经递质及受体表达的影响  

Effects of Jujuboside on Amino Acid Neurotransmitters and its Receptor Expression in the Brain of Syndrome Insomnia Old Rats

作　　者：张舜波[1] 王平[1] 田代志[1] 游秋云[1] 

机构地区：[1]湖北中医药大学,武汉430065

出　　处：《中国实验方剂学杂志》2014年第4期124-127,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072849)

摘　　要：目的:观察酸枣仁总皂苷对老年阴血亏虚证失眠大鼠脑组织谷氨酸(Glu)、γ-氨基丁酸(GABA)及GABA A受体表达的影响。方法:将Wistar大鼠随机分为空白对照组(等容生理盐水),模型组(老年阴血亏虚证失眠组,等容生理盐水),阳性对照组(安定0.18 mg·kg-1·d-1),酸枣仁总皂苷低、高剂量组(15,30 g·kg-1·d-1)。用D-半乳糖致亚急性衰老、环磷酰胺及氢化可的松致阴血亏虚,及自制改良多平台法剥夺快动眼睡眠(REM)48 h,制作阴血亏虚证候失眠老年大鼠模型。造模后各组灌胃给药3周,采用高效液相法检测脑皮质、海马部位Glu,GABA含量;免疫组化染色及RT-PCR法检测脑皮质、海马部位GABA A R的表达变化,并考察酸枣仁总皂苷对这些指标的影响。结果:与空白对照组比较,模型组大鼠脑皮质及海马Glu,GABA含量显著升高(P<0.01,P<0.05),且Glu/GABA增高;脑皮质及海马部位的GABA A R免疫化学累积吸光度明显较高(P<0.01);皮质部位GABA A R mRNA的表达均显著上调(P<0.01),经酸枣仁总皂苷干预后各指标明显下降(P<0.01,P<0.05)。结论:酸枣仁总皂苷治疗阴血亏虚证老年失眠的机制可能与减少脑内氨基酸毒性作用,下调大脑皮质及海马部位GABA A R的表达有关。Objective： To observe the effects of jujuboside （JBSD） on glutamic acid （Glu）, γ- aminobutyric acid （GABA） and γ-aminobutyric acid A receptor （ GABAA R） expression in the brain of syndrome insomnia old rats. Method： Wistar rats were randomly divided into 5 groups： blank control, syndrome model group, positive medicine group, high and low-dose JBSD treatment group. Multiple factors model were established with subacute senescence induced by D-galactose, Yin and blood-deficiency induced by cyclophosphamide and hydrocortisonum, and the rapid eye movement （REM） sleep deprivation （SD） induced by maded-self multiple platform method （MMPM）. After three weeks of administration, the levels of amino acid neurotransmitters Glu and GABA in cortex and hippocampus were detected by HPLC, and GABAAR expression were detected by immunohistochemical staining and retrovirus-polymerase chain reaction （RT-PCR）. After intervention of jujuboside, those indicators were studied. Result： Compared to environmental control group, in model group the content of Glu and GABA and the ratio of them increased in cortex and hypothalamus （P 〈 0. 01, P 〈 0.05 ） ,GABAAR immunochemistry absorbance was rose significantly （P 〈 0.01 ） in cortex and hippocampus, and GABAAR mRNA expression showed higher significantly （P 〈0.01） in cortex. While the indicators tended to the normal range after the treatment of JBSD （P 〈0.01, P 〈0.05）. Conclusion： JBSD can reduce the toxic effects of amino acids in brain, and downregulating GABAAR expression in the rat brain may be one of the mechanisms in treatment of the syndrome insomnia.

关 键 词：酸枣仁总皂苷 睡眠剥夺 氨基酸类神经递质 阴血亏虚证 

分 类 号：R285.5[医药卫生—中药学]