黄杨宁对大鼠胸主动脉血管环舒张作用的研究  

Vasorelaxant Effects of Cyclovirobuxine D on Rat Thoracic Aorta Rings

作　　者：余海滨[1,2] 王清照 沈俊岭[1,2] 任周新[1,2] 崔琳[1,4] 

机构地区：[1]河南中医学院第一附属医院,郑州450000 [2]河南省病毒性疾病中医药防治重点实验室,郑州450000 [3]河南省医药学校,河南开封475000 [4]国家中医药管理局中药药理三级科研实验室,郑州450000

出　　处：《中国实验方剂学杂志》2014年第4期149-153,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省教育厅科技攻关项目(2011A360022)

摘　　要：目的：研究黄杨宁（cyclovirobuxineD，CVB-D）对大鼠胸主动脉的舒张作用，并探讨其可能的作用机制。方法：分别采用氯化钾（KCl）和去氧肾上腺素（PE）预收缩血管，观察CVB-D（1×10^-5-6×10～mol·L^-1）对血管的舒张作用；将血管与6×10^-4mol-L-CVB-D预孵育，观察其对KCl或PE收缩血管作用的影响；观察不同抑制剂对CVB．D舒张大鼠离体血管环作用的影响。结果：在给药浓度范围内，CVB-D对KCl或PE预收缩血管环的舒张作用呈剂量依赖性；CVB-D对内皮完整血管环的舒张作用强于对去内皮血管环的舒张作用；CVB-D与血管预孵育可抑制KCl或PE引起的血管收缩；一氧化氮合成酶（NOS）抑制剂左旋硝基精氨酸甲酯（L-NAME）或选择性一氧化氮（NO）敏感的可溶性鸟苷酸环化酶（sGC）抑制剂ODQ可阻断CVB-D的血管舒张作用。结论：CVB-D对大鼠离体胸主动脉的血管舒张作用呈剂量依赖性，其舒张作用可能与一氧化氮一可溶性鸟苷酸环化酶一环磷酸鸟苷（NO-sGC-cGMP）途径相关。Objective： To observe the vasorelaxant effects of cyclovirobuxine D on isolated rat thoracic aorta rings and to investigate the possible mechanism. Method： The vasodilation of cyclovirobuxine D at various concentrations （range from 1 × 10^-5 to 6 ×10^-4mol.L-1 ） on potassium chloride （KC1） or phenylephrine （PE） - precontracted aorta rings was observed. KC1 or PE-induced contraction was also recorded after the aorta rings were preincubated with CVB-D at the concentration of 6 × 10^-4mol.L-1. When the aortic rings were incubated with different inhibitors, the effect of CVB-D on aortic rings was examined and investigated. Result： In KC1 or PE- precontracted aorta rings, CVB-D showed dose-dependent vasorelaxant effect at the given range of concentrations on the rings preconstricted by KC1 or PE. Futhermore, CVB-D exhibited stronger vasorelaxation effect on the aorta rings with intacted endothelium compared to the aorta rings denuded endothelium. Additionally, CVB-Dpreincubation could inhibit KC1 or PE-induced contraction, while nitric oxide NAME） or selected NO sensitive soluble guanylate cyclase （sGC） inhibitor 1H- （NO） Eli, synthase inhibitor （L- [2], [4] -oxadiazolo [4, 3-α] quinoxilin-l-one （ODQ） block the vasodilation effect of CVB-D. Conclusion： CVB-D has relaxation effect on rat isolated thoracic arterial NO-sGC-cGMP （nitric oxide-soluble rings in a dose-dependent manner, the relaxant effect may be related to the guanylate cyclase-cyclic guanosine monophosphate） pathway.

关 键 词：黄杨宁 胸主动脉 内皮 舒张作用 

分 类 号：R285.5[医药卫生—中药学]