葡萄白藜芦醇合酶基因转化水稻的研究  

Transforming rice (Oryza sativa L.) with a Vitis stilbene synthase gene

作　　者：刘聃璐 林拥军[1,2] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室 [2]华中农业大学国家植物基因研究中心(武汉),武汉430070

出　　处：《华中农业大学学报》2014年第2期8-14,共7页Journal of Huazhong Agricultural University

基　　金：国家“863”计划项目(2012AA10A303)

摘　　要：将来源于2个葡萄品种的白藜芦醇合酶基因以组成型表达的强启动子Ubiquitin驱动,并利用农杆菌介导的遗传转化引入粳稻品种中花11中。通过对单拷贝转基因家系基因表达量及白藜芦醇目标物的检测,发现大多数转基因家系的外源基因可稳定表达,但检测到的最终产物不是白藜芦醇,而是白藜芦醇苷(亦称云杉新苷),最高检出量达到了10.26μg/g,是野生型对照的1 000倍,推测这是在生成白藜芦醇的基础上,水稻内源的糖基转移酶作用的结果。虽然经抗病检测发现转基因植株并没有对稻瘟病菌表现出明显抗性,但是高含量的云杉新苷转基因家系可以作为提升水稻潜在营养价值的材料进行使用。Two resveratrol synthase genes from different Vitis spp. were isolated and replaced un der the control of Ubiquitin promoter, respectively. The vectors were then introduced into japonica rice cuhivar ZhongHua 11 through Agrobacterium-mediated gene transformation. Analyses of Southern blot and RT-PCR confirmed that the introduced genes were expressed stably in most of different single-copy gene transformants. Based on the HPLC-MS analysis, a major resveratrol derivative,piceid （resveratrol- glucoside） was detected in the leaves of the single-copy gene transformants with the highest content of 10.26μg/g fresh weight, 1 000-fold more than that of the wild type control. Resuls indicated that res- veratrol produced by the exogenously integrated gene might be metabolized into piceid by the endoge- nous glycosyltransferase. Although these transformants did not show significant resistance towards Magna- porthe grisea, they may have some beneficial effects on human health with the high content of Diceid.

关 键 词：白藜芦醇 云杉新苷 白藜芦醇合酶基因 水稻 

分 类 号：S511[农业科学—作物学] Q785[生物学—分子生物学]