香蕉与枯萎病菌4号小种互作过程中防御酶活性的变化  

Changes of defensive enzyme activities during the interactions of banana plantlets with Fusarium oxysporum f. sp. cubense race 4

作　　者：曾蕊[1,2] 陈琦光[2] 禄璐[2] 唐倩菲[2] 周而勋[2] 

机构地区：[1]上海农林职业技术学院园艺园林系,上海201600 [2]华南农业大学资源环境学院,广州510642

出　　处：《华中农业大学学报》2014年第2期61-64,共4页Journal of Huazhong Agricultural University

基　　金：国家现代农业(香蕉)产业技术体系建设专项(nycytx-33-06;CARS-32)

摘　　要：为探讨香蕉被枯萎病菌4号小种(Fusarium oxysporum f.sp.cubense race 4)侵染后防御酶活性的变化规律,采用伤根浸孢子液接种法,对粉蕉(ABB)和巴西蕉(AAA)的香蕉苗进行人工接种试验,测定香蕉苗与枯萎病菌互作过程中苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)5种防御酶的活性变化。结果表明:接种的香蕉苗体内PAL、POD、PPO和SOD酶活性都高于未接种的对照香蕉苗,且各种酶活性变化也比未接种的变化复杂,表明这4种酶在枯萎病菌侵染过程中积极参与了香蕉苗体内的抗病反应;但接种与未接种的香蕉苗体内CAT活性变化大致相似,表明CAT活性的变化与枯萎病菌的侵染无关。In order to investigate the changes of defensive enzyme activities in banana infected by Fusarium oxysporum f. sp. cubense race 4 （Foc 4）, the activities of phenylalanine ammonialyase （PAL）, peroxidase （POD）, polyphenol oxidase （PPO）, superoxide dismutase （SOD） and catalase （CAT） were determined during the interactions of Fenjiao （ABB） and Brazilian （AAA） banana plantlets with Foc 4 by using artificial inoculation method of dipping injure＆roots in conidial suspension. The results showed that the activities of PAL, POD, PPO and SOD were higher in the infected bananas than those in the corresponding non-infected controls, and the changes in activities of PAL, POD, PPO and SOD in infec- ted bananas were more complicated than those in the corresponding non-infected controls, which sugges- ted that these four enzymes actively participated in the resistant response in bananas during the infection of Foc 4. However, the variation trends of CAT activities in both infected and non-infected controls were general similar, indicating that the activity variation of CAT is not related to the infection of Foc 4.

关 键 词：香蕉 香蕉枯萎病菌 寄主一病原菌互作 防御酶 

分 类 号：S432.44[农业科学—植物病理学] S668.1[农业科学—农业昆虫与害虫防治]