不同脂肪含量饲料对吉富罗非鱼鱼种生长性能、脂蛋白脂酶活性及其基因表达的影响  

Effects of dietary lipid levels on growth performance,activity and gene expression of lipoprotein lipase in GIFT tilapia (Oreochromis niloticus) juvenile

作　　者：王爱民[1] 杨文平[1] 於叶兵[1] 吕富[1] 吴文静[1] 封功能[1] 王资生[1] 

机构地区：[1]盐城工学院海洋技术系/江苏省沿海池塘养殖生态重点实验室/江苏省沿海养殖鱼类饲料工程技术研究中心,盐城224051

出　　处：《华中农业大学学报》2014年第2期96-102,共7页Journal of Huazhong Agricultural University

基　　金：江苏省省属高校自然科学基础研究项目(08KJD240003);江苏省第四期"333高层次人才培养工程"第三层次培养对象资助项目

摘　　要：试验选用均质量(2.63±0.16 g)的吉富罗非鱼鱼种315尾,随机分成3组,每组3个重复,每个重复35尾,分别饲喂3.7%、7.7%和16.6%的低、中、高脂肪水平的等氮饲料,探讨不同脂肪含量饲料对吉富罗非鱼鱼种生长、肝体指数、脂肪沉积、脂蛋白脂酶(lipoprotein lipase,LPL)活性及其基因表达的影响,并初步探讨LPL基因表达与脂肪沉积的关联规律,试验周期90 d。结果显示:(1)饲喂高脂饲料的试验鱼肝体指数、脏体指数显著升高,肥满度无显著变化,肝脏与肌肉脂肪含量显著升高;(2)饲喂高脂肪饲料试验鱼的增重率显著下降、饲料系数下降显著;(3)LPL基因在吉富罗非鱼鱼种肝脏和肌肉中均有表达,但肝脏中LPL mRNA表达丰度显著高于肌肉;(4)在再投喂48 h内,在第6小时时,高脂肪试验组鱼肝脏LPL活性显著高于中、低脂肪试验组,但随后显著降低,显著低于3.7%试验组;7.7%和3.7%试验组鱼肝脏LPL活性呈现先升高后下降的趋势;(5)饲喂高脂饲料的试验鱼肝脏中LPL mRNA表达丰度最高,显著高于3.7%低脂试验组;(6)吉富罗非鱼肝体指数、肝脏和肌肉脂肪含量与肝脏LPL表达具有显著的线性正相关。结果表明,LPL mRNA在吉富罗非鱼肝脏和肌肉中表达具有组织特异性;高脂饲料显著抑制了吉富罗非鱼鱼种增重;高脂饲料显著促进了吉富罗非鱼肝脏LPL的分泌,诱导了其肝脏中LPL基因表达,促进肝脏与肌肉脂肪沉积;LPL基因参与调节吉富罗非鱼脂肪沉积。A 90-day study was conducted to investigate effects of dietary lipid levels on perform- ance, fat deposition, activity and expression of lipoprotein lipase （LPL） in GIFT tilapia （Oreochromis niloticus） juvenile,and to explore the correlation between LPL gene expression and fat deposition. 315 GIFT tilapia juveniles （average weight 2. 63± 0. 16 g） three replications, and fed with isonitrogenous diets were randomly divided into three groups with with different lipid levels of 3. 7% （low-lipid group） ,7.7% （middle-lipid group）and 16.6% （high-lipid group） by supplementing with 2%, 6% and 15 % fish oil as lipid source, respectively. The results showed. （1） the hepatosomatic index （HSI）, viscer- osomatic index （VSI） and lipid contents in liver and muscle increased with increasing dietary lipid level （P〈0. 05）, but there was a trends for condition factor （CF） with first increased then decreased （P〉0. 05）; （2）with increasing dietary lipid levels, the weight gain （WG） first increased, then de- creased, and feed conversion ratio （FCR） decreased（P〈0.05） ; （3）LPL gene was expressed in liver and muscle of GIFT tilapia, while the expression level in liver was significantly higher than that in muscle （P〈0. 05）; （4） during the 48 h post-refeeding period,liver LPL activity in high-lipid group was signifi- cantly higher than that in low-lipid and middle lipid group at 6 h, but significantly lower than that in low-lipid group at the 12 h,24 h and 48 h. There was a trend in low-lipid and middle-lipid group that liv- er LPL activity increased first and then decreased; （5） the expression of LPL was significantly higher in high-lipid group than that in low-lipid group （P〈0. 05） ; （6） there were a significant linear positive cor- relation between HSI,lipid contents in liver and muscle, and liver LPL expression in GIFT tilapia iuven- ile. The results indicated that LPL expression in GIFT tilapia was in a tissue specific pattern and liver wa

关 键 词：脂蛋白脂酶(LPL) 饲料脂肪水平 LPL基因表达 脂肪沉积 吉富罗非鱼 

分 类 号：S963[农业科学—水产养殖]