Optimization of Two-species Whole-cell Immobilization System Constructed with Marine-derived Fungi and Its Biological Degradation Ability  

由海洋微生物构建的双菌种全细胞固定化体系的优化及其生物降解性能研究(英文)

作　　者：陈慧英 王明霞 沈煜斌 姚善泾 

机构地区：[1]Department of Chemical and Biological Engineering,Zhejiang University

出　　处：《Chinese Journal of Chemical Engineering》2014年第2期187-192,共6页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(21036005);Scientific Technology Program of Zhejiang Province(2011C33016)

摘　　要：Mycelia pellet formed spontaneously in the process of cultivation was exploited as a biological carrier for whole-cell immobilization due to its unique structural characteristic. An innovative two-species whole-cell im- mobilization system was achieved by inoculating the marine-derived fungus Pestalotiopsis sp. J63 spores into cul- ture medium containing another fungus Penicillium janthinellum P1 pre-grown mycelia pellets for 2 days without any pretreatment. In order to evaluate the biological degradation capacity of this novel constructed immobilization system, the immobilized pellets were applied to treat paper mill effluent and decolorize dye Azure B. The use of the constructed immobilization system in the effluent resulted in successful and rapid biodegradation of numerous in- soluble fine fibers. The optimum conditions of immobilized procedure for maximum biodegradation capacity were determined using orthogonal design with biomass of P1 pellets 10 g （wet mass）, concentration of J63 spore 2x109 mlq, and immobilization time 2 d. The results demonstrate that immobilized pellets have more than 99% biodegradation capacity in a ten-hour treatment process. The kinetics of biodegradation fits the Michaelis-Menten equation well. Besides, the decolorization capability of immobilized pellets is more superior than that of P1 mycelia pellets. Overall, the present study offers a simple and reproducible way to construct a two-species whole-cell immobiliza- tion system for sewage treatment.Mycelia pellet formed spontaneously in the process of cultivation was exploited as a biological carrier for whole-cell immobilization due to its unique structural characteristic.An innovative two-species whole-cell immobilization system was achieved by inoculating the marine-derived fungus Pestalotiopsis sp.J63 spores into culture medium containing another fungus Penicillium janthinellum P1 pre-grown mycelia pellets for 2 days without any pretreatment.In order to evaluate the biological degradation capacity of this novel constructed immobilization system,the immobilized pellets were applied to treat paper mill effluent and decolorize dye Azure B.The use of the constructed immobilization system in the effluent resulted in successful and rapid biodegradation of numerous insoluble fine fibers.The optimum conditions of immobilized procedure for maximum biodegradation capacity were determined using orthogonal design with biomass of P1 pellets 10 g(wet mass),concentration of J63 spore 2×109ml?1,and immobilization time 2 d.The results demonstrate that immobilized pellets have more than 99%biodegradation capacity in a ten-hour treatment process.The kinetics of biodegradation fits the Michaelis-Menten equation well.Besides,the decolorization capability of immobilized pellets is more superior than that of P1 mycelia pellets.Overall,the present study offers a simple and reproducible way to construct a two-species whole-cell immobilization system for sewage treatment.

关 键 词：whole-cell immobilization mycelia pellet Pestalotiopsis sp. Penicillium janthinellum biodegradation kinetics biological wastewater treatment 

分 类 号：Q814.2[生物学—生物工程]