Cellular prion protein imaging analysis with aptamer-labeled Ru(bpy)_3^(2+)-doped silica nanoparticles  

作　　者：Wei Wang Xiao Li Yan Lei Zhan Fei Leng Xiao Xi Yang Cheng Zhi Huang 

机构地区：[1]Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences,Southwest University [2]College of Chemistry and Chemical Engineering, Southwest University

出　　处：《Chinese Science Bulletin》2014年第2期147-153,共7页

基　　金：supported by the National Basic Research Program of China(2011CB933600)

摘　　要：A new type of highly selective aptamer-labeled fluorescent silica nanoparticles[Apt-tris(2,20-bipyridyl)ruthenium(II)@SiO2NPs]were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris(2,20-bipyridyl)ruthenium(II)(Ru(bpy)32?)as the source of the fluorescence for cellular prion protein imaging.Investigations showed that the newly prepared Ru(bpy)32?@SiO2NPs possessed superior advantages of strong fluorescence,low toxicity,and easy surface modification for bioconjugation.Cell imaging experiments indicated that AptRu(bpy)32?@SiO2NPs had great tendency to human bone marrow neuroblastoma cells(SK-N-SH cells),since they can express large amount of prion protein on the surface of the cell,while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins.A new type of highly selective aptamer-labeled fluorescent silica nanoparticles [Apt-tris（2,2＇-bipyri- dyl）ruthenium（II）@SiO2 NPs] were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris（2,2＇-bipyri- dyl）ruthenium（II） （Ru（bpy）2＋） as the source of the fluorescence for cellular prion protein imaging. Investigations showed that the newly prepared Ru（bpy）32＋@Si02 NPs possessed superior advantages of strong fluorescence, low toxicity, and easy surface modification for bioconjugation. Cell imaging experiments indicated that Apt- Ru（bpy）32＋@SiO2 NPs had great tendency to human bone marrow neuroblastoma cells （SK-N-SH cells）, since they can express large amount of prion protein on the surface of the cell, while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins.

关 键 词：二氧化硅纳米粒子 三联吡啶钌 神经细胞 成像分析 朊蛋白 标记 适体 SIO2纳米粒子 

分 类 号：O657.3[理学—分析化学]