拟南芥LRR-RLKs亚家族蛋白RLK6的亚细胞定位及RLK6的组织表达  

Subcellular Localization of RLK6,a Protein of LRR-RLK Subfamily and Tissue Expression Pattern of the RLK6 in Arabidopsis

作　　者：阿依江.哈拜克 杨敏[1,3] 韩玉珍[1] 

机构地区：[1]中国农业大学生物学院植物生理生化国家重点实验室,北京100193 [2]新疆医科大学药学院,乌鲁木齐830011 [3]河南科技大学农学院,河南洛阳471003

出　　处：《西北植物学报》2014年第1期1-6,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30670192);教育部博士点基金(20120008110018)

摘　　要：以拟南芥(Arabidopsis thaliana)为材料,运用RT-PCR技术扩增得到了富含亮氨酸的类受体蛋白激酶(LRR-RLKs)亚家族基因RLK6,构建了RLK6与绿色荧光蛋白基因(GFP)融合表达载体并转化拟南芥,用激光共聚焦扫描显微镜观察转基因植物细胞表明:RLK6蛋白定位于细胞膜上;将RLK6-GFP在原生质体中进行瞬时表达,进一步证实了RLK6蛋白定位于细胞膜上。构建了RLK6启动子(2 063bp)融合GUS报告基因的载体并转化拟南芥,对转基因植株进行组织化学染色分析表明:RLK6在拟南芥的幼苗、根、花、角果等组织中都有表达,花中表达量较高,尤其是在雄蕊中特异高表达,而在茎、莲座叶和干种子中几乎没有表达。RT-PCR分析结果与GUS组织化学染色的结果一致。研究推测,RLK6可能在花器官生长发育或相关生理过程的信号转导中发挥作用。The full length coding sequence without the TAA stop codon of LRR-RLKs gene （RLK6） of Ar- abidopsis thaliana was cloned by RT-PCR and fused with green fluorescent protein （GFP） gene to construct a plant expression vector. The stable Arabiclopsis transformants were observed using confocal laser scanning microscope. The result indicated that RLK6-GFP fusion protein was localized in the plasma mem- branes. Consistent location was obtained by the experiment of RLK6-GFP transient expression in living Arabidopsis protoplasts. To visualize the temporal and spatial expression patterns RLK6 in plant tissue, a 2 063 bp promoter fragment of RLK6 was fused with the GUS gene to generate transgenic plants. GUS ac- tivity was detected in seedlings, roots and flowers, especially in anther,but hardly detected in stems, rosette leaves and dry seeds. Consistent result was obtained by RT-PCR analysis. Overall, these data implied that RLK6 might involve in the signaling pathway of flower organs development or related processes.

关 键 词：拟南芥 LRR—RLKs 亚细胞定位 启动子 组织表达 

分 类 号：Q789[生物学—分子生物学]