AtRabD2b共抑制植株死亡茎段的显微和超微结构观察  

Microstructural and Ultrastructural Observation of the Dead Stem Sections in Arabidopsis thaliana RabD2b-cosuppressed Plant

作　　者：王芳[1] 刘超[1] 刘克德[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《西北植物学报》2014年第1期7-12,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(30623012)

摘　　要：采用树脂包埋技术,以AtRabD2b共抑制植株死亡发生茎段为实验材料,制备半薄切片和超薄切片,观察突变体茎段死亡的细胞学特征。结果表明:(1)共抑制植株茎细胞的死亡首先在表皮细胞层中发现,然后向切片圆周两侧以及内侧细胞蔓延。(2)共抑制植株茎细胞出现染色质边缘化、叶绿体内囊体片层膜数目减少、细胞器成分被液泡吞噬等异常现象。这些细胞学特征暗示共抑制植株茎段发生了细胞程序性死亡,由此推断AtRabD基因对拟南芥茎顶端细胞的生长有重要的维持作用。The semi-thin sections or ultra-thin sections were made by resin embedding technique using the upper region of the dying stem of AtRabD2b-cosuppressed plant. The sections were observed using light microscope or transmission electron microscopy （TEM） to study the cellular process of the stem death. The results indicated that： （1）The death was firstly initiated from several periphery epidermal cells, and then spread from cell to cell both laterally and longitudinally throughout the whole stem section. （2）Several ab- normal phenomenons such as the condensation and peripheral location of chromatin, the decrease of the numbers of internal thylakoid membranes of chloroplast and the autophagy of organelles by vacuole were observed. These features revealed that PCD occurs in the dying cells of the mutant apical stem. It also suggested that AtRabD genes are essential for the cell survival in the apical stem.

关 键 词：拟南芥 AtRabD2b 死亡发生茎段 细胞程序性死亡 

分 类 号：Q246[生物学—细胞生物学] Q255