天山雪莲未知蛋白基因的克隆及其耐铝特性分析  

Clone and Aluminum Tolerance Analysis of Unknown Gene fromSaussurea involucrata Kar.et Kir.

作　　者：王海霞[1] 张林华[1] 杨晶[1] 张亚敏[1] 张伟[1] 王爱英[2] 胡鸢雷[3] 祝建波[2] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]石河子大学农业生物技术重点实验室,新疆石河子832003 [3]北京大学生命科学学院,北京100871

出　　处：《西北植物学报》2014年第1期32-39,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31160049)

摘　　要：从天山雪莲叶片低温诱导的cDNA文库中克隆了一个未知蛋白基因(SikSD-82)cDNA全长序列。生物信息学分析表明SikSD-82基因共编码262个氨基酸,具有小麦铝诱导蛋白Wali7的保守结构域,属Gn_AT_Ⅱ家族的可溶性蛋白;其氨基酸序列与蓖麻的茎部特异性表达蛋白相似性最高为60.89%;进化树分析显示,该蛋白与番茄的茎部特异性表达蛋白关系最近。亚细胞定位结果表明,SikSD-82蛋白主要定位于细胞核。在铝胁迫条件下,转SikSD-82烟草幼苗根的伸长明显优于野生型,根部铝离子和丙二醛含量均显著低于野生型烟草,转SikSD-82烟草显示出较强的耐铝性。A unknown function protein gene SikSD-82 was cloned from Saussurea involucrata Kar. et Kir. eDNA library under cold-stress. Physieoehemical properties and molecular features of SikSD-82 were pre- dicted by bioinformatic analysis showed that SikSD-82 gene encoding a stable protein with 262 amino acids,which contains a conserved WaliT（wheat aluminum induced protein） ,a member of Gn_AT_ 1I super- family, had significant identity of 60.89 ~ with stem-specific proteins from Ricinus communis. Phylogenetic analysis indicated that SikSD-82 protein was most closely related to Solanum lycopersicum stem specific protein. The subcellular localization of SikSD-82 was found mostly in the nucleus . Under aluminum stress,SikSD-82 transgenic plant accumulate less A1 and MDA concentration in roots than that of wild- type plants,and their roots are longer than that of wild-type plants~ ,which shows that SikSD-82 transgenic plants have more aluminum tolerance than that of wild-type plants.

关 键 词：天山雪莲 铝胁迫 基因克隆 亚细胞定位 功能分析 

分 类 号：Q785[生物学—分子生物学] Q789