氯化镁预处理对美人蕉根部质膜H^+-ATPase活性与农田废水硝态氮吸收效率的影响  

Effects of Magnesium Chloride Pretreatment on the Plasma Membrane H^+-ATPase Activity and Nitrate Absorption Efficiency of Canna generalis in Farmland Wastewater

作　　者：刘昂[1] 吴文卫[2] 赵磊[2] 陈奇[1] 白晓华[2] 陈丽梅[1] 

机构地区：[1]昆明理工大学生命科学与技术学院生物工程技术研究中心,昆明650500 [2]云南省环境科学研究院,昆明650034

出　　处：《西北植物学报》2014年第1期128-134,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：云南省科技计划(社会事业发展专项2010CA001)

摘　　要：以湿地挺水植物七叶红色大花美人蕉(Cana generalis Bailey)幼苗为实验材料,在温室中用150μmol/L氯化镁溶液预处理12h,然后置于硝态氮(NO3--N)浓度为10mg/L的农田废水中处理,考察氯化镁预处理对美人蕉吸收农田废水中硝态氮效率的影响,以及处理期间根部质膜H+-ATPase活性、H+-泵活性、14-3-3蛋白与质膜H+-ATPase相互作用。结果显示:经过150μmol/L氯化镁溶液预处理12h后,美人蕉吸收农田废水中硝态氮的效率比对照增加11%;与没有预处理的植株相比,氯化镁预处理美人蕉根中质膜H+-ATPase和H+-泵的活性均显著提高,质膜H+-ATPase和14-3-3蛋白的相互作用显著增强。研究表明,氯化镁能够通过增强质膜H+-ATPase与14-3-3蛋白的相互作用来提高质膜H+-ATPase的活性,从而增加美人蕉对硝态氮的吸收效率。In this study, the seedlings of the wetland emergent plant, Canna generalis Bailey, were used as the experimental materials. Pretreatment of the seedlings with 150/~mol/L MgC12 was conducted under the greenhouse conditions for 12 h. Subseqently, treatment of the seedlings with farmland wastewater contai- ning 10 mg/L nitrate （NOa--N） was performed to investigate the effects of MgClz pretreatment on the ni- trate absorption efficiency,the plasma membrane H^＋-ATPase and H^＋ pump activities and the interaction of plasma membrane H^＋-ATPase and 14-3-3 protein of C. generalis in farmland wastewater. The results showed that pretreatment of 150 /amol/L MgC12 for 12 h increased the nitrate absorption efficiency of C. generalis Bailey in farmland wastewater by 11%. Compared with those in the roots of the plants without MgC12 pretreatment,both the plasma membrane H^＋-ATPase and H^＋ pump activities in C. generalis roots with Mgc pretreatment were significantly elevated. MgC12 pretreatment also enhanced plasma membrane H^＋-ATPase and 14-3-3 protein interaction in C. generalis roots. These results suggested that MgCI2 pre- treatment increased the Plasma membrane H^＋-ATPase activity by enhancing its interaction with 14-3-3 protein,thereby increasing the nitrate absorption efficiency of C. generalis in farmland wastewater.

关 键 词：美人蕉 氯化镁 农田废水 硝态氮 质膜H^+-ATPASE 

分 类 号：Q945.79[生物学—植物学] Q948.116