九龙藤黄酮对H_2O_2诱导乳鼠心肌细胞凋亡的抑制作用  

Inhibitory effects of Bauhinia championii flavones on cadiocytes apoptosis induced by H_2O_2

作　　者：林炜鑫[1] 孙瑶[1] 苏福海[1] 方瑜[1] 简洁[1] 

机构地区：[1]桂林医学院,广西桂林541004

出　　处：《中国新药与临床杂志》2014年第1期39-43,共5页Chinese Journal of New Drugs and Clinical Remedies

基　　金：广西自然科学基金课题(2012GXNSFAA053148);广西教育厅课题(201103yb094)

摘　　要：目的研究九龙藤黄酮(BCF)对H_2O_2诱导的乳鼠心肌细胞凋亡的保护作用及机制。方法原代培养乳鼠心肌细胞,采用100 p,mol-L^(-1)H_2O_2孵育16 h建立心肌细胞凋亡模型,随机建立正常组、模型组、维生素C(终浓度100 mg·L^(-1))预处理组及不同浓度BCF(终浓度分别50、100、200 mg·L^(-1))预处理组。各给药组均于造模前给药孵育2h。MTT法检测细胞活力,Annexin V-FITC/PI双染法测定心肌细胞凋亡率。ELISA法测定细胞培养上清液肿瘤坏死因子(TNF)-α,分光光度法测定总抗氧化活力(T-AOC),免疫组化方法观察Bcl-2、Bax蛋白的表达。结果与模型组相比,不同浓度BCF预处理组的心肌细胞存活率、T-AOC和Bcl-2蛋白表达均显著增高(P<0.01或P<0.05);而INF-α、Bax蛋白表达、心肌细胞凋亡率均显著下降(P<0.01或P<0.05)。与维生素C组相比,BCF各浓度组无显著差异(P>0.05)。结论 BCF可抑制H_2O_2引起的乳鼠心肌细胞凋亡,其作用机制可能与抗脂质过氧化、降低TNF-α、上调Bcl-2及下调Bax蛋白表达有关。AIM To observe the effects of flavones from Bauhinia championii （BCF） on H2O2-induced cardiomyocytes apoptosis in neonatal rats and its mechanisms. METHODS Peroxidative damage model was induced by adding 100 μmol·L^-1 H2O2 in neonatal rat cardiomyocytes for 16 h. The rat cardiomycytes was randomly divided into normal group, model group, vitamin C （100 mg·L^-1） and BCF （50, 100, 200 mg·L^-1） preconditioning groups. BCF and vitamin C were administrated for 2 h prior to HzO2 treatment. Cell viabilities were measured by MTT assay. Annexin V- FITC/PI staining was used to detect cell apoptosis. Tumor necrosis factor alpha （TNF-α） and total antioxidant activity （T-AOC） was determined by ELISA, and the protein expression of Bcl- 2 and Bax was observed by immunohistochemistry. RESULTS Compared with the model group, BCF pretreatment increased cell viability, T-AOC and the expression of Bcl-2 （P 〈 0.01 or P 〈 0.05）, while decreased cardiomyocytes apoptosis and the expression of TNF- α and Bax. There was no significant difference （P 〉 0.05） between the vitamin C group and the BCF groups. CONCLUSION BCF inhibits H2O2- induced cardiomyocytes apoptosis, which might be mediated by decreasing lipid peroxidation, reducing TNF-α, up-regulating the expression of Bcl-2 and down-regulating Bax.

关 键 词：九龙藤黄酮 过氧化氢 肌细胞 心脏 细胞凋亡 

分 类 号：R961[医药卫生—药理学]