大鼠重复注射米托蒽醌热敏脂质体产生ABC现象  

Repeated injection of mitoxantrone containing thermosensitive liposomes in rat induced ABC phenomenon

作　　者：田伟[1] 张兰 魏娜 李蝉 倪蓓蓓 赵曦 李春雷[1,2] 

机构地区：[1]河北医科大学药学院,河北石家庄050017 [2]石药集团中奇制药技术(石家庄)有限公司,河北省制剂工程技术研究中心,新型药物制剂与辅料国家重点实验室,河北石家庄050035

出　　处：《药学学报》2014年第2期256-259,共4页Acta Pharmaceutica Sinica

基　　金：国家重点基础研究发展计划(973计划)资助项目(2010CB735603)

摘　　要：考察大鼠重复注射米托蒽醌热敏脂质体是否产生加快血液清除现象(accelerated blood clearance phenomenon,ABC phenomenon)。液质联用测定大鼠血浆中米托蒽醌浓度,并对血浆样品中抗聚乙二醇(polyethylene glycol,PEG)IgM抗体进行酶联免疫吸附剂测定(enzyme linked immunosorbent assay,ELISA)的检测。结果显示,第2次给药后药物清除速率变快,给药前血浆中抗PEG IgM抗体在第2次给药后被迅速中和。证明大鼠重复注射米托蒽醌热敏脂质体会产生ABC现象。To investigate whether accelerated blood clearance （ABC） phenomenon could be induced after repeated injection of mitoxantrone thermosensitive liposomes, LC-MS/MS and enzyme linked immunosorbent assay （ELISA） were used to measure the concentration of mitoxantrone and the anti-polyethylene glycol （PEG） IgM levels in rat plasma, separately. The drug was rapidly cleared away after the second administration. The anti-PEG IgM was detected after the first dose which was neutralized quickly after the second dose. It is proved that repeated administration of mitoxantrone thermosensitive liposomes in rat caused the ABC phenomenon.

关 键 词：米托蒽醌 热敏脂质体 液质联用 药代动力学 ABC现象 

分 类 号：R917[医药卫生—药物分析学]