藏药佐太对CYP1A2和NAT2的活性及蛋白和mRNA表达的影响  

Effect of Tibetan medicine Zuotai on the activity,protein and mRNA expression of CYP1A2 and NAT2

作　　者：李向阳[1] 刘永年[1] 李永平[1] 朱俊博[1] 姚星辰[1] 李永芳[1] 杨梅[1] 袁明[1] 范雪汝[1] 尹月淼[1] 

机构地区：[1]青海大学医学院药学系,青海西宁810001

出　　处：《药学学报》2014年第2期267-272,共6页Acta Pharmaceutica Sinica

基　　金：教育部新世纪优秀人才支持计划(NCET-10-0917);国家自然科学基金资助项目(30960457);青海省自然科学基金资助项目(2013-Z-909)

摘　　要：藏药佐太为藏语译音，也称为佐塔，“佐”意为煮煎，“太”意为灰或炭，译成中文是“煮炼之灰”的意剧”。佐太具有调血、生肌健脾、滋补强身的功效，在藏医药的使用中有悠久的历史，应用面广，使用量大；其配制工艺独特烦琐，用料复杂，是在金属汞中加以多种辅助药物，经过洗、涤、煮等多道炮制工艺，To study the effect of Tibetan medicine Zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2, three different doses （1.2, 3.8 and 12 mg ·kg^-1） of Zuotai were administrated orally to rats once a day or once daily for twelve days, separately. Rats were administrated orally caffeine （CF） on the second day after Zuotai administration, and the urine concentration of CF metabolite 5-acetylamino-6-formylamino-3- methyl-uracil （AFMU）, 1-methyluric acid （1U）, 1-methylxanthine （IX）, 1, 7-dimethylxanthine （17U） at 5 h after study drug administration was determined by RP-HPLC. The activity of CYP1A2 and NAT2 was evaluated by the ratio of metabolites （AFMU＋IX＋IU）/17U and the ratio of AFMU/（AFMU＋IX＋IU）, respectively. The protein and mRNA expression of CYP1A2 and NAT2 were determined by ELISA and RT-PCR method, respectively. After single administration of Zuotai 3.8 mg ·kg^-1 and repeated administration of Zuotai 3.8 and 12 mg ·kg^-1, the activity of CYP1A2 and NAT2 decreased significantly compared with control group and there was no significant difference between other dose group and control group. The protein expression of CYP1A2 was significant lower than that in control group after repeated administration of Zuotai 12 mg ·kg^-1, and the mRNA expression of CYP1A2 decreased significantly compared with that of control group after single administration of Zuotai 3.8 mg ·kg^-1 and repeated admistration of Zuotai 12mg ·kg^-1, separately. The protein expression of NAT2 decreased significantly compared with that of control group after single and repeated administration of Zuotai 3.8 mg ·kg^-1, respectively, and the mRNA expression of CYP1A2 decreased significantly compared with control group after single administration of Zuotai 3.8 mg ·kg^-1 This study found that Tibetan medicine Zuotai had significant effect on the activity, protein and mRNA expression of CYPIA2 and NAT2.

关 键 词：藏药 佐太 CYP1A2 NAT2 

分 类 号：R963[医药卫生—微生物与生化药学]