地锦草有效部位对红色毛癣菌细胞膜合成及MEP、SUB基因表达的影响  

Action of Euphorbia humifusa effective fraction on membrane biosynthesis and the gene expression of proteases MEP and SUB of Trichophyton rubrum

作　　者：李治建 赵明月 古力娜.达吾提 斯拉甫.艾白 

机构地区：[1]新疆维吾尔自治区维吾尔医药研究所,新疆乌鲁木齐830049 [2]新疆维吾尔医方剂学实验室,新疆乌鲁木齐830049 [3]新疆维吾尔自治区维吾尔医医院,新疆乌鲁木齐830049

出　　处：《药学学报》2014年第2期273-276,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81060370;81160557);新疆维吾尔自治区自然科学基金资助项目(2011211B52);新疆维吾尔自治区公益性科研院所基本科研业务费专项资金项目(KY2013104)

摘　　要：地锦草为大戟科植物地锦（Euphorbia humifusa Willd）或斑地锦（Euphorbia maculataL）的干燥全草，是中医、维吾尔医常用药材，临床主要用于治疗手癣、体癣、足癣、花斑癣、银屑病.以往研究表明，地锦草提取物及其有效部位（含40％黄酮和16％鞣酸）对浅部致病真菌有较强的抑制作用。This study is to investigate the effect of Euphorbia humifusa effective fraction （EHEF） on the CYP51 enzyme activity, the lanosterol content and the MEP, SUB gene expression of Trichophyton rubrum. Trichophyton rubrum was treated by EHEF for 7 days at 26 ~C. The activity of CYP51 enzyme of Trichophyton rubrum in the cell membrane was determined by using ELISA kit, and the lanosterol content was investigated by using high performance liquid chromatography （HPLC）, and the MEP, SUB gene expression of Trichophyton rubrum was detected with the reverse transcription polymerase chain reaction （RT-PCR） method. Results showed that EHEF can decrease the membrane CYP51 enzyme activity, and it also can accumulate the fungal lanosterol in a dose-dependent manner, and it also can decrease the gene expression of MEP and SUB. The antifungal mechanism of EHEF may be related to the inhibition on CYP51 enzyme activity, and to the effects on fungal cell membrane ergosterol biosynthesis. It may also play an antifungal effect by inhibiting the MEP, SUB gene expression of fungal proteases.

关 键 词：地锦草有效部位 红色毛癣菌 基质金属蛋白酶 枯草菌素蛋白酶 CYP51酶 

分 类 号：R963[医药卫生—微生物与生化药学]